Mertk deficiency affects macrophage directional migration via disruption of cytoskeletal organization.
Mertk deficiency affects macrophage directional migration via disruption of cytoskeletal organization.
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DOI：
10.1371/journal.pone.0117787
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Lu Q
中科院分区：
文献类型：
--
作者：
Tang Y;Wu S;Liu Q;Xie J;Zhang J;Han D;Lu Q;Lu Q
Mertk belongs to the Tyro3, Axl and Mertk (TAM) family of receptor tyrosine kinases, and plays a pivotal role in regulation of cytoskeletal rearrangement during phagocytosis. Phagocytosis by either professional or non-professional phagocytes is impaired in the Mertk deficient individual. In the present study, we further investigated the effects of Mertk mutation on peritoneal macrophage morphology, attachment, spreading and movement. Mertk-mutated macrophages exhibited decreased attachment, weak spreading, loss of spindle-like body shape and lack of clear leading and trailing edges within the first few hours of culture, as observed by environmental scanning electron microscopy. Time-lapse video photography recording showed that macrophage without Mertk conducted mainly random movement with oscillating swing around the cell body, and lost the directional migration action seen on the WT cells. Western blotting showed a decreased phosphorylation of focal adhesion kinase (FAK). Immunocytochemistry revealed that actin filaments and dynamic protein myosin II failed to concentrate in the leading edge of migrating cells. Microtubules were localized mainly in one side of mutant cell body, with no clear MTOC and associated radially-distributed microtubule bundles, which were clearly evident in the WT cells. Our results suggest that Mertk deficiency affects not only phagocytosis but also cell shape and migration, likely through a common regulatory mechanism on cytoskeletons.
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DOI：
10.1084/jem.20041447
发表时间：
2004-12-20
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
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Finnemann SC
DOI：
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发表时间：
2002-11-25
期刊：
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作者：
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DOI：
10.1083/jcb.200708093
发表时间：
2007-12-17
期刊：
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影响因子：
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作者：
Owen KA;Pixley FJ;Thomas KS;Vicente-Manzanares M;Ray BJ;Horwitz AF;Parsons JT;Beggs HE;Stanley ER;Bouton AH
通讯作者：
Bouton AH
DOI：
10.1083/jcb.75.3.666
发表时间：
1977-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Malech HL;Root RK;Gallin JI
通讯作者：
Gallin JI
DOI：
10.1073/pnas.73.3.867
发表时间：
1976-01-01
影响因子：
11.1
作者：
OSBORN, M;WEBER, K
通讯作者：
WEBER, K