11-Ketotestosterone is a major androgen produced in porcine adrenal glands and testes
11-Ketotestosterone is a major androgen produced in porcine adrenal glands and testes
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11-酮睾酮是猪肾上腺和睾丸中产生的主要雄激素
DOI：
10.1016/j.jsbmb.2021.105847
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Ida Takano
中科院分区：
文献类型：
--
作者：
Yazawa Takashi;Sato Takahiro;Nemoto Takahiro;Nagata Sayaka;Imamichi Yoshitaka;Kitano Takeshi;Sekiguchi Toshio;Uwada Junsuke;Islam Mohammad Sayful;Mikami Daisuke;Nakajima Ikuyo;Takahashi Satoru;Khan Md. Rafiqul Islam;Suzuki Nobuo;Umezawa Akihiro;Ida Takano
猪类固醇激素谱具有一些独特的特征。我们以前研究了人类和小鼠类固醇生成使用类固醇生成细胞来源于间充质干细胞（MSC）。为了研究猪类固醇生成，我们从猪皮下前脂肪细胞（PSPA细胞）诱导类固醇生成细胞，其来源于MSC。使用cAMP，腺病毒介导的类固醇生成因子-1（SF-1）/肾上腺4-结合蛋白（Ad 4 BP）的引入诱导PSPA细胞分化为性类固醇产生细胞。SF-1/Ad 4 BP的导入也诱导了醛酮还原酶1C 1（AKR 1C 1）基因的表达。猪AKR 1C 1具有17β-羟基类固醇脱氢酶活性，可将雄烯二酮和11-酮雄烯二酮转化为睾酮（T）和11-酮睾酮（11 KT）。此外，分化的细胞表达羟基类固醇11β-脱氢酶2（HSD 11B 2）并产生11 KT。HSD 11B 2在睾丸间质细胞和肾上腺皮质中表达。11 KT存在于未成熟的雄性和雌性猪的血浆中，其中雄性猪的水平略高。雄性猪的T水平要高得多。值得注意的是，在雌性猪中，11 KT水平比T水平高>10倍。然而，阉割改变了11 KT和T血浆配置文件中的雄性猪接近的女性。11 KT诱导猪血管内皮细胞内皮型一氧化氮合酶（eNOS）的表达。这些结果表明，11 KT在猪肾上腺和睾丸中产生，并可能通过eNOS表达调节心血管功能。
Porcine steroid hormone profiles have some unique characteristics. We previously studied human and murine steroidogenesis using steroidogenic cells-derived from mesenchymal stem cells (MSCs). To investigate porcine steroidogenesis, we induced steroidogenic cells from porcine subcutaneous preadipocytes (PSPA cells), which originate from MSCs. Using cAMP, adenovirus-mediated introduction of steroidogenic factor-1 (SF-1)/adrenal 4-binding protein (Ad4BP) induced the differentiation of PSPA cells into sex steroid-producing cells. Introducing SF-1/Ad4BP also induced the aldo-keto reductase 1C1 (AKR1C1) gene. Porcine AKR1C1 had 17β-hydroxysteroid dehydrogenase activity, which converts androstenedione and 11-ketoandrostenedione into testosterone (T) and 11-ketotestosteorne (11KT). Furthermore, differentiated cells expressed hydroxysteroid 11β-dehydrogenase 2 (HSD11B2) and produced 11KT. HSD11B2 was expressed in testicular Leydig cells and the adrenal cortex. 11KT was present in the plasma of both immature male and female pigs, with slightly higher levels in the male pigs. T levels were much higher in the male pigs. It is noteworthy that in the female pigs, the 11KT levels were >10-fold higher than the T levels. However, castration altered the 11KT and T plasma profiles in the male pigs to near those of the females. 11KT induced endothelial nitric oxide synthase (eNOS) in porcine vascular endothelial cells. These results indicate that 11KT is produced in porcine adrenal glands and testes, and may regulate cardiovascular functions through eNOS expression.
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影响因子：
4.1
作者：
T. Yazawa;Shinya Kawabe;Y. Inaoka;R. Okada;T. Mizutani;Yoshitaka Imamichi;Yunfeng Ju;Yukiko Yamazaki;Y. Usami;Mayu Kuribayashi;A. Umezawa;K. Miyamoto
通讯作者：
T. Yazawa;Shinya Kawabe;Y. Inaoka;R. Okada;T. Mizutani;Yoshitaka Imamichi;Yunfeng Ju;Yukiko Yamazaki;Y. Usami;Mayu Kuribayashi;A. Umezawa;K. Miyamoto
使用雄激素受体介导的反式激活评估 17β-羟基类固醇脱氢酶活性。
DOI：
--
发表时间：
2020
期刊：
The Journal of Steroid Biochemistry and Molecular Biology
影响因子：
--
作者：
Yazawa;T.;Imamichi;Y.;Uwada;J.;Sekiguchi;T.;Mikami;D.;Kitano;T.;Ida;T. Sato;T.;Nemoto;T.;Nagata;S.;Khan;R.;Takahashi;S.;Ushikubi;F.;Taniguchi;A.;Suzuki;N. and Umezawa;A.
通讯作者：
A.
人内皮细胞中的雄激素受体。
DOI：
10.1530/joe-14-0611
发表时间：
2015-03
期刊：
The Journal of endocrinology
影响因子：
--
作者：
Torres-Estay V;Carreño DV;San Francisco IF;Sotomayor P;Godoy AS;Smith GJ
通讯作者：
Smith GJ
影响因子：
4.1
作者：
Pretorius, Elzette;Ark, Wiebke;Storbeck, Karl-Heinz
通讯作者：
Storbeck, Karl-Heinz
一氧化氮和cGMP抗血管平滑肌增殖作用的分子机制是什么？
影响因子：
37.8
作者：
F. Murad
通讯作者：
F. Murad