A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging.
A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging.
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DOI：
10.1002/dvg.20758
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发表时间：
2011-07
期刊：
影响因子：
1.5
通讯作者：
Aizawa, Shinichi
中科院分区：
文献类型：
--
作者：
Shioi, Go;Kiyonari, Hiroshi;Abe, Takaya;Nakao, Kazuki;Fujimori, Toshihiko;Jang, Chuan-Wei;Huang, Cheng-Chiu;Akiyama, Haruhiko;Behringer, Richard R.;Aizawa, Shinichi
实时成像是可视化活细胞并监测其在发育过程中的行为的重要工具。该技术需要多种小鼠报告细胞系，每种都独特地表达荧光蛋白。在这里，我们开发了一种R26 R-RG报告小鼠系，其分别从Rosa 26位点在细胞核和质膜中有条件地同时表达mCherry和EGFP。mCherry核定位和EGFP膜定位的强度和分辨率被证明足以用于在胎儿支持细胞中普遍且特异性地表达RG（mCherry和EGFP）的胚胎的活体成像。有条件的R26 R-RG报告小鼠系应该是一个有用的工具，在不同的细胞群体中同时标记细胞核和细胞膜。
Live-imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R-RG reporter mouse line that conditionally and simultaneously expresses mCherry and EGFP in nuclei and plasma membranes, respectively, from the Rosa26 locus. The intensity and resolution of mCherry nuclear localization and EGFP membrane localization were demonstrated to be sufficient for live-imaging with embryos that express RG (mCherry and EGFP) ubiquitously and specifically in fetal Sertoli cells. The conditional R26R-RG reporter mouse line should be a useful tool for labeling nuclei and membranes simultaneously in distinct cell populations.
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影响因子：
5.4
作者：
Trichas, Georgios;Begbie, Jo;Srinivas, Shankar
通讯作者：
Srinivas, Shankar
影响因子：
2.7
作者：
Kisseberth, WC;Brettingen, NT;Sandgren, EP
通讯作者：
Sandgren, EP
影响因子：
3.5
作者：
Cormack, BP;Valdivia, RH;Falkow, S
通讯作者：
Falkow, S
影响因子：
1.5
作者：
Stewart, M. David;Jang, Chuan-Wei;Hong, Nicholas W.;Austin, Anthony P.;Behringer, Richard R.
通讯作者：
Behringer, Richard R.
DOI：
10.1073/pnas.0504750102
发表时间：
2005-10-11
影响因子：
11.1
作者：
Akiyama, H;Kim, JE;de Crombrugghe, B
通讯作者：
de Crombrugghe, B