Simultaneous and sensitive measurement of nicotine, cotinine, trans-3'-hydroxycotinine and norcotinine in human plasma by liquid chromatography-tandem mass spectrometry.
Simultaneous and sensitive measurement of nicotine, cotinine, trans-3'-hydroxycotinine and norcotinine in human plasma by liquid chromatography-tandem mass spectrometry.
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DOI：
10.1016/j.jchromb.2009.08.033
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发表时间：
2009-11-01
期刊：
影响因子：
--
通讯作者：
Huestis MA
中科院分区：
文献类型：
--
作者：
Shakleya DM;Huestis MA
开发并充分验证了同时定量人血浆中尼古丁、可替宁、反式-3 '-羟基可替宁和去甲可替宁的LCMSMS方法。对潜在的内源性和外源性干扰进行了广泛评价，并通过降低分析物浓度来确定定量限。尼古丁和可替宁的分析范围为1-500 ng/mL，反式-3 '-羟基可替宁和去甲可替宁的分析范围为5-500 ng/mL。所有分析物的平均试验内和试验间分析回收率在101.9%至116.8%之间，试验内和试验间不精密度小于11% RSD：在试验线性范围内的三个不同浓度下评价了参数。所有分析物的提取效率≥ 70%。该经验证的方法可用于测定人血浆中的尼古丁和代谢物，以支持尼古丁生物标志物对神经元系统介导认知和情感过程的作用的研究，并区分主动、被动和环境暴露。
An LCMSMS method for the simultaneous quantification of nicotine, cotinine, trans-3’-hydroxycotinine and norcotinine in human plasma was developed and fully validated. Potential endogenous and exogenous interferences were extensively evaluated and limits of quantification were determined by decreasing analyte concentration. Analytical ranges were 1–500 ng/mL for nicotine and cotinine, 5–500 ng/mL for trans-3’-hydroxycotinine and norcotinine. Mean intra- and inter-assay analytical recovery were between 101.9 and 116.8%, and intra- and inter-assay imprecision were less than 11% RSD for all analytes: parameters were evaluated at three different concentrations across the linear range of the assay. Extraction efficiency was ≥ 70% for all analytes. This validated method is useful for determination of nicotine and metabolites in human plasma to support research on the role of nicotine biomarkers on neuronal systems mediating cognitive and affective processes and to differentiate active, passive and environmental exposure.
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影响因子：
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