Naturally occurring precore/core region mutations of hepatitis B virus genotype C related to hepatocellular carcinoma.
Naturally occurring precore/core region mutations of hepatitis B virus genotype C related to hepatocellular carcinoma.
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DOI：
10.1371/journal.pone.0047372
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Kim BJ
中科院分区：
文献类型：
--
作者：
Kim DW;Lee SA;Hwang ES;Kook YH;Kim BJ
先前的研究已经证明乙型肝炎病毒（HBV）感染中存在几种不同类型的突变，这些突变与肝病的进展有关。然而，很少有报道详细介绍前核心/核心（preC/C）区域的突变频率和突变模式，这些突变频率和突变模式是基于临床状态和HBeAg血清状态。本研究的目的是探讨 C 基因型 HBV 慢性感染患者的 preC/C 突变与临床严重程度或 HBeAg 血清状态之间的关系。共有 70 名韩国慢性患者参与了这项研究，其中包括 35 名肝细胞癌 (HCC) 患者。通过直接测序分析 HBV 基因分型和前核心/核心突变。所有患者均被证实患有C基因型感染。 C区的突变以非随机方式分布。特别是MHC II类限制区的突变被发现与HCC显着相关。六种（preC-W28*、C-P5H/L/T、C-E83D、C-I97F/L、C-L100I 和 C-Q182K/*）和七种类型（preC-W28*、preC-G29D、C-D32N/H、C-E43K、C-P50A/H/Y、C-A131G/N/P 和发现前 C/C 区的突变（C-S181H/P）分别与 HCC 相关并影响 HBeAg 血清状态。总之，我们的数据表明，C 区，特别是 MHC II 类限制区的 HBV 变异可能导致 C 基因型慢性感染者的 HCC 进展。此外，我们在韩国慢性人群中发现了几种不同的 preC/C 突变，这些突变影响 C 基因型感染患者的 HCC 临床状态和 HBeAg 血清状态。
Previous studies have proved the presence of several distinct types of mutations in hepatitis B virus (HBV) infections, which are related to the progression of liver disease. However, few reports have detailed the mutation frequencies and mutation patterns in the precore/core (preC/C) region, which are based on the clinical status and HBeAg serostatus. Our aim in this study is to investigate the relationships between the preC/C mutations and clinical severity or HBeAg serostatus from patients chronically infected with HBV genotype C. A total of 70 Korean chronic patients, including 35 with hepatocellular carcinoma (HCC), participated in this study. HBV genotyping and precore/core mutations were analyzed by direct sequencing. All patients were confirmed to have genotype C infections. Mutations in the C region were distributed in a non-random manner. In particular, mutations in the MHC class II restricted region were found to be significantly related to HCC. Six (preC-W28*, C-P5H/L/T, C-E83D, C-I97F/L, C-L100I and C-Q182K/*) and seven types (preC-W28*, preC-G29D, C-D32N/H, C-E43K, C-P50A/H/Y, C-A131G/N/P and C-S181H/P) of mutations in the preC/C region were found to be related to HCC and to affect the HBeAg serostatus, respectively. In conclusion, our data indicated that HBV variants in the C region, particularly in the MHC class II restricted region, may contribute to the progress of HCC in chronic patients infected with genotype C. In addition, we found several distinct preC/C mutations in the Korean chronic cohort, which affect the clinical status of HCC and HBeAg serostatus of patients infected with genotype C.
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影响因子：
12.7
作者：
Kim, Daniel;Lyoo, Kwang Soo;Smith, Davey;Hur, Wonhee;Hong, Sung Woo;Sung, Pil Soo;Yoon, Seung Kew;Mehta, Sanjay
通讯作者：
Mehta, Sanjay
影响因子：
29.4
作者：
Bruix, J;Castells, A;Rodes, J
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Rodes, J
影响因子：
25.7
作者：
Lee, Seoung-Ae;Kim, Kijeong;Kim, Bum-Joon
通讯作者：
Kim, Bum-Joon
影响因子：
13.5
作者：
Baptista, M;Kramvis, A;Kew, MC
通讯作者：
Kew, MC
影响因子：
5.8
作者：
Kumar, S;Tamura, K;Nei, M
通讯作者：
Nei, M