Evolution of several cytoskeletal proteins of astrocytes in primary culture: Effect of prenatal alcohol exposure
Evolution of several cytoskeletal proteins of astrocytes in primary culture: Effect of prenatal alcohol exposure
复制标题
原代培养中星形胶质细胞几种细胞骨架蛋白的进化：产前酒精暴露的影响
作者：
R. Sáez;M. Burgal;J. Renau‐Piqueras;A. Marqués;C. Guerri
在目前的工作中，我们分析了，使用免疫印迹和免疫荧光技术，在原代培养的星形胶质细胞的发展过程中的几个细胞骨架蛋白的演变。产前暴露于酒精对这些蛋白质的影响也进行了评估。微管蛋白α-微管蛋白从4到7天减少约47%，之后其含量几乎保持不变。免疫荧光研究还表明，α-微管蛋白的含量在培养的第4天较高。这种荧光的增加与在间期星形胶质细胞中发现的球状颗粒的存在一致，并且用抗α-和抗β-微管蛋白染色。这些结构仅出现在增殖细胞中。胶质细胞酸性蛋白（GFAP）和波形蛋白被分析为中间丝（IF）蛋白。细胞骨架制剂中的GFAP在14天内定期增加，然后在第21天减少。相反，波形蛋白在整个培养期间显示出逐渐增加。荧光研究揭示了GFAP和vimentin. In从大鼠产前暴露于乙醇中获得的星形胶质细胞的IF分布模式之间的一些差异，在整个培养期间发现所有研究的细胞骨架蛋白的量减少。在这些细胞中，还观察到细胞骨架的显著紊乱。当在"体内"全脑制备物中研究这种蛋白质时，也发现了培养的星形胶质细胞中乙醇诱导的GFAP减少。
In the present work we have analyzed, using immunoblotting and immunofluorescence techniques, the evolution of several cytoskeletal proteins during the development of astrocytes in primary culture. The effect of prenatal exposure to alcohol on these proteins was also evaluated. Microtubular protein α-tubulin decreased approximately 47% from 4 to 7 days after which its content remained practically constant. Immunofluorescence studies showed also that the content of α-tubulin was greater at day 4 of culture. This increase in fluorescence was coincident with the presence of globular particles which were found in interphase astrocytes and stained with both anti α- and anti-β-tubulin. These structures appeared only in proliferating cells. Glial fibrillary acidic protein (GFAP) and vimentin were analyzed as intermediate filament (IF) proteins. GFAP, in cytoskeletal preparations, increased regularly for 14 days followed by a decrease to day 21. In contrast, vimentin showed a progressive increase throughout the entire culture period. Fluorescence studies revealed some differences between the IF distribution patterns of GFAP and vimentin.In astrocytes obtained from rats prenatally exposed to ethanol, decreases in the amounts of all the cytoskeletal proteins studied were found during the entire culture period. In these cells a striking disorganization of cytoskeleton was also observed. The alcohol-induced decrease of GFAP in cultured astrocytes was also found when this protein was studied in preparations from whole brain developed “in vivo”.
影响因子：
2.9
作者：
A. Streissguth;F. Bookstein;P. Sampson;H. Barr
通讯作者：
A. Streissguth;F. Bookstein;P. Sampson;H. Barr
乙醛与蛋白质结合的功能后果。
DOI：
--
发表时间：
1987
期刊：
Alcohol and alcoholism (Oxford, Oxfordshire). Supplement.
影响因子：
--
作者：
Tuma,DJ;Sorrell,MF
通讯作者：
Sorrell,MF
影响因子：
56.9
作者：
RAKIC, P
通讯作者：
RAKIC, P