Bacillus subtilis CK3 used as an aquatic additive probiotics enhanced the immune response of crayfish Procambarus clarkii against newly identified Aeromonas veronii pathogen
Bacillus subtilis CK3 used as an aquatic additive probiotics enhanced the immune response of crayfish Procambarus clarkii against newly identified Aeromonas veronii pathogen
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枯草芽孢杆菌CK3用作水产添加剂益生菌增强小龙虾克氏原螯虾对新发现的维氏气单胞菌病原体的免疫反应
DOI：
10.1111/are.15571
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发表时间：
2021-09
影响因子：
2
通讯作者：
Qiuning Liu
中科院分区：
文献类型：
--
作者：
Hui Yang;Meng Zhang;Tongwei Ji;Yingying Zhang;Wenzhi Wei;Qiuning Liu
为明确克氏原螯虾暴发性疾病的病原，从克氏原螯虾肝胰脏中分离到一株优势菌。经生化鉴定和16S rDNA序列测定，鉴定为Veronii气单胞菌。人工感染克氏原螯虾的症状与自然感染克氏原螯虾的症状相似。该菌对诺氟沙星、环丙沙星、头孢他啶、头孢呋辛、头孢曲松敏感，对青霉素、氨苄西林、苯唑西林、哌拉西林耐药。检测到肠毒素基因（act、altandast）和溶血毒素基因（hlyAandaerA）等毒力基因。感染后部分肝小管间连接消失，刷状缘出现空泡，线粒体增大。还检测到以前鉴定的枯草芽孢杆菌CK3对阿氏曲霉的拮抗作用。枯草芽孢杆菌CK3的上清液对阿氏不动杆菌有明显的杀菌作用。枯草芽孢杆菌CK3浸泡后，肝胰腺中抗氧化酶和免疫相关酶活性显著高于对照。在养殖水体中添加B.subtilisCK3可显著降低由Veronican感染引起的累积死亡率。这些结果表明，B.subtilisCK3可以作为一种水添加剂，以提高免疫反应的P. clarkii对新鉴定的A. veronii。本研究为克氏原螯虾细菌性病害的防治提供了新的途径，也为克氏原螯虾的健康养殖提供了技术支持。
To identify the aetiology of the disease outbreak in culturedProcambarus clarkii, a dominant bacteria strain from the diseased crayfish hepatopancreas was isolated. It was identified asAeromonas veroniibased on biochemical identification and 16S rDNA sequencing. The symptoms of artificially infected crayfish were similar to those of naturally infectedP.clarkii. TheA.veroniiwas sensitive to norfloxacin, ciprofloxacin, ceftazidime, cefuroxime and ceftriaxone antibiotics, while resistant to penicillin, ampicillin, oxacillin and piperacillin. Virulence genes such as the enterotoxin genes (act,altandast) and haemolytic toxin genes (hlyAandaerA) were detected. AfterA.veroniiinfection, the connections between some liver tubules disappeared, the vacuoles appeared in the brush border and the mitochondria were enlarged. The antagonistic action of previous identifiedB.subtilisCK3 againstA.veroniiwas also detected. The supernatant ofB.subtilisCK3 exhibits a significant bactericidal effect onA.veronii. AfterB.subtilisCK3 immersion, the antioxidant enzymes and immune‐related enzyme activities in hepatopancreas were significantly higher than control. The accumulative mortality caused by infection ofA.veroniican be significantly reduced by addingB.subtilisCK3 into the aquatic water. These results demonstrated thatB.subtilisCK3 could act as a water additive to improve the immune response ofP.clarkiiagainst newly identifiedA.veronii. The present study will provide a new way for the prevention and control of crayfish bacterial diseases and also provide technical support for the healthy cultivation ofP.clarkii.
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影响因子：
4.5
作者：
Ha Thanh Dong;Vuong Viet Nguyen;Rodkhum, Channarong
通讯作者：
Rodkhum, Channarong
DOI：
10.1007/s10482-012-9786-z
发表时间：
2012
期刊：
Antonie van Leeuwenhoek
影响因子：
--
作者：
K. Sreedharan;R. Philip;I. Singh
通讯作者：
K. Sreedharan;R. Philip;I. Singh
影响因子：
4.4
作者：
Nawaz, Mohamed;Sung, Kidon;Steele, Roger
通讯作者：
Steele, Roger
影响因子：
4.7
作者：
H. Sung;Yi-Hsiu Lin;C. Hsiao
通讯作者：
H. Sung;Yi-Hsiu Lin;C. Hsiao
DOI：
10.1017/s002531540005222x
发表时间：
1988-05-01
影响因子：
1.2
作者：
CACECI, T;NECK, KF;SIS, RF
通讯作者：
SIS, RF