Fluoride stimulates the accumulation of inositol phosphates, increases intracellular free calcium, and inhibits parathyroid hormone release in dispersed bovine parathyroid cells.
Fluoride stimulates the accumulation of inositol phosphates, increases intracellular free calcium, and inhibits parathyroid hormone release in dispersed bovine parathyroid cells.
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氟化物刺激磷酸肌醇的积累，增加细胞内游离钙，并抑制分散的牛甲状旁腺细胞中甲状旁腺激素的释放。
DOI：
10.1210/endo-122-6-2833
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发表时间：
1988
期刊：
影响因子：
4.8
通讯作者：
J. M. Mcghee
中科院分区：
文献类型：
--
作者：
D. Shoback;J. M. Mcghee
The stimulation of polyphosphoinositide (PPI) turnover is associated with cellular activation and hormone secretion in numerous systems. GTP-binding proteins appear to couple receptors to phospholipase-C-mediated PPI breakdown. We assessed the effects of fluoride, an activator of GTP-binding proteins, on inositol phosphate accumulation, intracellular free Ca2+ [(Ca2+)i], cAMP content, and PTH release in dispersed bovine parathyroid cells. Sodium fluoride (5-30 mM) produced marked dose-dependent increases in inositol phosphates. With anion exchange HPLC, we confirmed that 30 mM fluoride stimulated a rapid increase in 1,4,5-inositol trisphosphate, a potent Ca2+-mobilizing compound. Using the Ca2+-sensitive probe fura-2, we determined that 30 mM fluoride increased [Ca2+]i from 339 +/- 9 to 650 +/- 39 nM (n = 8) within 30-60 sec at 1 mM extracellular Ca2+. After the depletion of extracellular Ca2+ by the addition of 1 mM EGTA, 30 mM fluoride increased [Ca2+]i 45 +/- 9% (n = 4), indicating that fluoride can mobilize intracellular Ca2+ stores. Fluoride (1-30 mM) also inhibited PTH release in dose-dependent fashion. Fluoride (30 mM) produced 72.8 +/- 4.2% suppression of maximal low Ca2+-stimulated PTH release comparable to the 83.7 +/- 3.7% inhibition by 2.0 mM extracellular Ca2+. Since changes in both [Ca2+]i and cAMP regulate PTH release, we measured the effect of fluoride on intracellular cAMP. Fluoride did not detectably change basal cAMP content, but it reduced forskolin-stimulated increases in cAMP. We conclude that fluoride may activate at least two GTP-dependent processes in parathyroid cells, resulting in PPI breakdown and cAMP accumulation. While both may contribute to the fluoride-induced suppression of PTH release, our findings suggest that the stimulation of PPI turnover leads to inhibition of PTH secretion.
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牛甲状旁腺细胞的刺激-分泌耦合。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Nemeth,EF;Wallace,J;Scarpa,A
通讯作者：
Scarpa,A
DOI：
10.1016/s0021-9258(18)67502-7
发表时间：
1986-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Martin;S. Bajjalieh;D. Lucas;J. Kowalchyk
通讯作者：
T. Martin;S. Bajjalieh;D. Lucas;J. Kowalchyk
5-羟色胺刺激苍蝇唾液腺无细胞系统中磷酸肌醇的产生。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Litosch,I;Wallis,C;Fain,JN
通讯作者：
Fain,JN
DOI：
10.1073/pnas.79.16.4888
发表时间：
1982-08
影响因子：
11.1
作者：
P. Sternweis;A. Gilman
通讯作者：
P. Sternweis;A. Gilman
鸟嘌呤核苷酸结合调节蛋白在钙动员激素水解肝细胞磷脂酰肌醇 4,5-二磷酸和细胞钙控制中的作用。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Blackmore,PF;Bocckino,SB;Waynick,LE;Exton,JH
通讯作者：
Exton,JH