Recorded scan times can limit the accuracy of sedimentation coefficients in analytical ultracentrifugation.
Recorded scan times can limit the accuracy of sedimentation coefficients in analytical ultracentrifugation.
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DOI：
10.1016/j.ab.2013.02.011
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发表时间：
2013-06-01
影响因子：
2.9
通讯作者：
Schuck P
中科院分区：
文献类型：
--
作者：
Zhao H;Ghirlando R;Piszczek G;Curth U;Brautigam CA;Schuck P
我们报告的系统和大的不准确性，在记录的时间从分析ultracenthoge的数据文件，导致高估的沉降系数高达10%。这远远超过了先前考虑的因素，导致该参数的不确定性，并有显着的衍生参数，如流体动力学形状和摩尔质量估计的影响。该误差的来源目前尚不清楚，但我们发现它在不同的仪器上是定量一致的，随着转子速度的增加而增加。此外，它的发生似乎与使用制造商的最新数据采集软件有关，该软件在我们的一些实验室中使用了近两年。许多最近公布的沉降系数可能需要重新审查。通过将操作系统提供的文件时间戳与数据文件中记录的扫描时间进行比较，可以轻松识别问题。因此，我们在SEDFIT中实现了一个例程，它可以自动检查数据文件，提醒用户存在重大差异，并相应地纠正扫描时间。这消除了记录的扫描时间中的误差。
We report systematic and large inaccuracies in the recorded elapsed time in data files from the analytical ultracentrifuge, leading to overestimates of the sedimentation coefficients of up to 10%. This far exceeds previously considered factors contributing to the uncertainty in this parameter, and has significant ramifications for derived parameters, such as hydrodynamic shape and molar mass estimates. The source of this error is at present unknown, but we found it to be quantitatively consistent across different instruments, increasing with rotor speed. Furthermore, its occurrence appears to correlate with the use of the latest data acquisition software from the manufacturer, in use in some of our laboratories for nearly two years. Many of the recently published sedimentation coefficients may need to be re-examined. The problem can be easily recognized by comparing the file time-stamps provided by the operating system with the elapsed scan times recorded within the data files. We therefore implemented a routine in SEDFIT that can automatically examine the data files, alert the user to significant discrepancies, and correct the scan times accordingly. This eliminates errors in the recorded scan times.
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影响因子：
3.4
作者：
Schuck, P
通讯作者：
Schuck, P
DOI：
10.1007/s00249-009-0514-1
发表时间：
2009-10
影响因子：
2
作者：
Brown, Patrick H.;Balbo, Andrea;Schuck, Peter
通讯作者：
Schuck, Peter
影响因子：
3.4
作者：
Schuck, P;Perugini, MA;Schubert, D
通讯作者：
Schubert, D
DOI：
10.1073/pnas.44.7.671
发表时间：
1958-01-01
影响因子：
11.1
作者：
MESELSON, M;STAHL, FW
通讯作者：
STAHL, FW
影响因子：
4.8
作者：
Zhao, Huaying;Balbo, Andrea;Brown, Patrick H.;Schuck, Peter
通讯作者：
Schuck, Peter