Radioimmunoassay of atrial natriuretic factor: human plasma levels.

Radioimmunoassay of atrial natriuretic factor: human plasma levels.
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心房钠尿因子的放射免疫测定:人血浆水平。

DOI:
10.1016/0006-291x(85)90452-8
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发表时间:
1985
影响因子:
3.1
通讯作者:
Huy Ong
Huy Ong
中科院分区:
生物学4区
文献类型:
--
作者:
P. Larose;S. Meloche;P. D. Souich;A. Delean;Huy Ong

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建立了一种简便、灵敏的人血浆心钠素(ANF)放射免疫测定法。从商业来源获得兔抗血清。用十八烷基硅胶柱从血浆中提取ANF,回收率为78.7%。血浆提取物的HPLC显示存在一个对应于其低分子量形式的ANF免疫反应性峰。人血浆ANF从直立位的8.0 ± 2.2增加到向下位的20.0 ± 5.9 fmol/ml(n = 6)(p < 0.005)。
A simple and sensitive radioimmunoassay procedure has been developed for the determination of atrial natriuretic factor (ANF) in human plasma. The rabbit antiserum was obtained from a commercial source. ANF was extracted from plasma using an octadecasilyl silica cartridge with a recovery of 78.7%. HPLC of the plasma extract showed the presence of one immunoreactive peak of ANF correponding to its low molecular weight form. Plasma ANF in humans increased from 8.0 ± 2.2 in upright position to 20.0 ± 5.9 fmol/ml (n = 6) in downward position (p < 0.005).
大鼠下丘脑、心房和血浆中的心房钠尿因子:通过特异性放射免疫测定法测定。
DOI: 10.1016/0006-291x(84)91606-1
发表时间: 1984
影响因子: 3.1
作者:
Tanaka,I;Misono,KS;Inagami,T
通讯作者: Inagami,T