Actin-based movement of Listeria monocytogenes: actin assembly results from the local maintenance of uncapped filament barbed ends at the bacterium surface.
Actin-based movement of Listeria monocytogenes: actin assembly results from the local maintenance of uncapped filament barbed ends at the bacterium surface.
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基于肌动蛋白单核细胞增生李斯特菌的运动：肌动蛋白组装是由于细菌表面的固定细丝末端的局部维护而产生的。
DOI：
10.1083/jcb.130.2.331
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发表时间：
1995-07
影响因子：
7.8
通讯作者：
PANTALONI, D
中科院分区：
文献类型：
--
作者：
MARCHAND, JB;MOREAU, P;PAOLETTI, A;COSSART, P;CARLIER, MF;PANTALONI, D
已经使用最初由Theriot等人开发的爪蟾卵的细胞质提取物研究了单核细胞增生李斯特菌的基于肌动蛋白的运动性的热力学基础（Theriot，J.A.，J. Rosenblatt，D. A. Portnoy，P. J. Goldschmidt-Clermont，and T. J·米奇森1994. Cell. 76：505-517）作为体外无细胞系统。大部分（75%）的肌动蛋白被发现未聚合的提取物。未组装的肌动蛋白（12 μ M）的量由T β 4Xen（20 μ M）和profilin（5 μ M）的螯合功能来解释，倒刺末端被封端。李斯特菌的运动并没有被超过99%的内源性profilin的消耗所消除。ActA富含脯氨酸的序列不太可能是profilin的靶点。所有的数据都支持这样的观点，即李斯特菌后部的肌动蛋白组装是由于一个因素导致稳态的局部转变，保持细丝未加帽，结合到细菌的表面，而倒刺末端在大量细胞质中加帽。运动由能量差控制（即，临界浓度的差异），因此需要恒定的ATP供应和培养基中存在有倒钩的端部加帽的F-肌动蛋白以缓冲高浓度的游离G-肌动蛋白。膜组分的作用由以下事实证明：（a）李斯特菌运动可以在富含膜的高速离心提取物的重悬颗粒中重建;（B）内源性囊泡的基于肌动蛋白的运动性，其表现出与李斯特菌相同的火箭运动，可以在提取物中观察到。
The thermodynamic basis for actin-based motility of Listeria monocytogenes has been investigated using cytoplasmic extracts of Xenopus eggs, initially developed by Theriot et al. (Theriot, J. A., J. Rosenblatt, D. A. Portnoy, P. J. Goldschmidt-Clermont, and T. J. Mitchison. 1994. Cell. 76:505-517) as an in vitro cell-free system. A large proportion (75%) of actin was found unpolymerized in the extracts. The amount of unassembled actin (12 microM) is accounted for by the sequestering functions of T beta 4Xen (20 microM) and profilin (5 microM), the barbed ends being capped. Movement of Listeria was not abolished by depletion of over 99% of the endogenous profilin. The proline-rich sequences of ActA are unlikely to be the target of profilin. All data support the view that actin assembly at the rear of Listeria results from a local shift in steady state due to a factor, keeping filaments uncapped, bound to the surface of the bacterium, while barbed ends are capped in the bulk cytoplasm. Movement is controlled by the energetic difference (i.e., the difference in critical concentration) between the two ends of the filaments, hence a constant ATP supply and the presence of barbed end capped F-actin in the medium are required to buffer free G-actin at a high concentration. The role of membrane components is demonstrated by the facts that: (a) Listeria movement can be reconstituted in the resuspended pellets of high speed-centrifuged extracts that are enriched in membranes; (b) Actin-based motility of endogenous vesicles, exhibiting the same rocketing movement as Listeria, can be observed in the extracts.
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影响因子：
2.9
作者：
HEISS, SG;COOPER, JA
通讯作者：
COOPER, JA
影响因子：
64.8
作者：
FORSCHER, P;LIN, CH;THOMPSON, C
通讯作者：
THOMPSON, C
DOI：
10.1083/jcb.118.6.1421
发表时间：
1992-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hartwig JH
通讯作者：
Hartwig JH
影响因子：
--
作者：
BUSS, F;TEMMGROVE, C;JOCKUSCH, BM
通讯作者：
JOCKUSCH, BM
影响因子：
4.8
作者：
ISAMBERT, H;VENIER, P;CARLIER, MF
通讯作者：
CARLIER, MF