Fluostatins M-Q Featuring a 6-5-6-6 Ring Skeleton and High Oxidized A-Rings from Marine Streptomyces sp. PKU-MA00045.
Fluostatins M-Q Featuring a 6-5-6-6 Ring Skeleton and High Oxidized A-Rings from Marine Streptomyces sp. PKU-MA00045.
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氟他汀 M-Q 具有 6-5-6-6 环骨架和来自海洋链霉菌的高氧化 A 环。
DOI：
10.3390/md16030087
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发表时间：
2018-03-09
期刊：
影响因子：
5.4
通讯作者：
Ma M
中科院分区：
文献类型：
--
作者：
Jin J;Yang X;Liu T;Xiao H;Wang G;Zhou M;Liu F;Zhang Y;Liu D;Chen M;Cheng W;Yang D;Ma M
海洋放线菌中的芳香聚酮类化合物因其独特的结构和强大的生物活性而受到越来越多的关注。与陆地上的同类化合物相比，海洋芳香族聚酮类化合物的发现较少，其结构和生物多样性也远未得到充分的研究。在这项研究中，我们采用基于pcr的基因组挖掘方法在我们的海洋细菌收集中发现芳香聚酮。从一株“阳性”链霉菌PKU-MA00045中发现了5种新的非典型氨环素类药物氟他汀M-Q(1-5)，具有独特的6-5-6-6环骨架。通过综合光谱分析对氟他汀M-Q（1-5）的结构进行了解析，对含a环氧化程度最高的氟他汀P(4)的晶体结构进行了Cu Kα辐射x射线衍射分析。与已发表的16种氟他汀类似物相比，氟他汀M-Q（1-5）在C-7和C-4上含有不同的甲氧基，丰富了海洋放线菌芳香族聚酮的结构多样性。Streptomyces sp. PKU-MA00045基因组测序揭示了氟他汀M-Q（1-5）的生物合成基因簇，该基因簇与已知的氟他汀基因簇相比包含不同的基因和基因组织，有助于研究所有氟他汀类药物独特的6-5-6-6环状骨架的生物合成。
Aromatic polyketides from marine actinomycetes have received increasing attention due to their unusual structures and potent bioactivities. Compared to their terrestrial counterparts, marine aromatic polyketides have been less discovered and their structural and biological diversities are far from being fully investigated. In this study, we employed a PCR-based genome mining method to discover aromatic polyketides in our marine bacteria collection. Five new atypical angucyclinones, fluostatins M–Q (1–5) featuring a unique 6-5-6-6 ring skeleton, were discovered from one “positive” Streptomyces sp. PKU-MA00045. The structures of fluostatins M–Q (1–5) were elucidated based on comprehensive spectroscopic analyses and the crystallographic structure of fluostatin P (4), which contains the most oxidized A-ring, was solved by X-ray diffraction analysis with Cu Kα radiation. Compared to the published 16 fluostatin analogues, fluostatins M–Q (1–5) contained a different methoxy group attached at C-7 and hydroxy group attached at C-4, enriching the structural diversity of aromatic polyketides from marine actinomycetes. Genome sequencing of Streptomyces sp. PKU-MA00045 revealed the biosynthetic gene cluster of fluostatins M–Q (1–5), which contained different genes and gene organizations compared to known fluostatin gene clusters, facilitating the investigation of the biosynthesis of the unique 6-5-6-6 ring skeleton in all fluostatins.
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影响因子：
5.1
作者：
Xie P;Ma M;Rateb ME;Shaaban KA;Yu Z;Huang SX;Zhao LX;Zhu X;Yan Y;Peterson RM;Lohman JR;Yang D;Yin M;Rudolf JD;Jiang Y;Duan Y;Shen B
通讯作者：
Shen B
影响因子：
15
作者：
He, HY;Ding, WD;Carter, GT
通讯作者：
Carter, GT
影响因子：
2.1
作者：
Janso, Jeffrey E.;Haltli, Brad A.;Eustaquio, Alessandra S.;Kulowski, Kerry;Waldman, Abraham J.;Zha, Li;Nakamura, Hitomi;Bernan, Valerie S.;He, Haiyin;Carter, Guy T.;Koehn, Frank E.;Balskus, Emily P.
通讯作者：
Balskus, Emily P.
影响因子：
11.9
作者：
Jensen PR;Moore BS;Fenical W
通讯作者：
Fenical W
影响因子：
18.3
作者：
Das, Abhirup;Khosla, Chaitan
通讯作者：
Khosla, Chaitan