TaClpS1, negatively regulates wheat resistance against Puccinia striiformis f. sp. tritici.
TaClpS1, negatively regulates wheat resistance against Puccinia striiformis f. sp. tritici.
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TaClpS1负调控小麦对条锈菌的抗性。白藓
DOI：
10.1186/s12870-020-02762-0
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发表时间：
2020-12-10
影响因子：
5.3
通讯作者：
Guo J
中科院分区：
文献类型：
--
作者：
Yang Q;Islam MA;Cai K;Tian S;Liu Y;Kang Z;Guo J
细胞内蛋白质的降解在植物对逆境胁迫的反应中起着重要的作用。ClpS 1和E3泛素连接酶分别在酪蛋白水解肽酶（Clp）蛋白酶途径和26 S蛋白酶体系统中作为用于选择靶底物的衔接子。目前，E3泛素连接酶在植物对病原体的免疫应答中的作用已被很好地确定。然而，ClpS 1在植物对病原体的免疫应答中的作用仍然未知。本文对小麦ClpS 1（TaClpS 1）基因进行了研究，结果表明该基因编码161个氨基酸，含有一个保守的ClpS结构域和一个叶绿体转运肽（1-32 aa）。TaClpS 1在小麦原生质体中瞬时表达时，被发现特异性地定位于叶绿体中。TaClpS 1基因在小麦中的转录水平在小麦条锈菌侵染过程中受到显著诱导。小麦种（Pst）通过病毒诱导的基因沉默（VIGS）敲低TaClpS 1基因，小麦对Pst的抗性增强，同时伴随着过敏反应（HR）、活性氧（ROS）积累、TaPR 1和TaPR 2表达的增加，吸器数量、侵染菌丝长度和侵染面积的减少。此外，TaClpS 1在本氏烟草中的异源表达增强了寄生疫霉的感染。这些结果表明TaClpS 1负调控小麦对Pst的抗性。在线版本包含补充材料，可通过10.1186/s12870-020-02762-0获得。
The degradation of intracellular proteins plays an essential role in plant responses to stressful environments. ClpS1 and E3 ubiquitin ligase function as adaptors for selecting target substrates in caseinolytic peptidase (Clp) proteases pathways and the 26S proteasome system, respectively. Currently, the role of E3 ubiquitin ligase in the plant immune response to pathogens is well defined. However, the role of ClpS1 in the plant immune response to pathogens remains unknown. Here, wheat (Triticum aestivum) ClpS1 (TaClpS1) was studied and resulted to encode 161 amino acids, containing a conserved ClpS domain and a chloroplast transit peptide (1–32 aa). TaClpS1 was found to be specifically localized in the chloroplast when expressed transiently in wheat protoplasts. The transcript level of TaClpS1 in wheat was significantly induced during infection by Puccinia striiformis f. sp. tritici (Pst). Knockdown of TaClpS1 via virus-induced gene silencing (VIGS) resulted in an increase in wheat resistance against Pst, accompanied by an increase in the hypersensitive response (HR), accumulation of reactive oxygen species (ROS) and expression of TaPR1 and TaPR2, and a reduction in the number of haustoria, length of infection hypha and infection area of Pst. Furthermore, heterologous expression of TaClpS1 in Nicotiana benthamiana enhanced the infection by Phytophthora parasitica. These results suggest that TaClpS1 negatively regulates the resistance of wheat to Pst. The online version contains supplementary material available at 10.1186/s12870-020-02762-0.
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DOI：
10.1111/j.1365-313x.2010.04112.x
发表时间：
2010-03
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Santner A;Estelle M
通讯作者：
Estelle M
影响因子：
4.5
作者：
Chen, W. Q.;Wu, L. R.;Wang, B. T.
通讯作者：
Wang, B. T.
影响因子：
7.2
作者：
Holzberg, S;Brosio, P;Pogue, GP
通讯作者：
Pogue, GP
影响因子：
11.6
作者：
Czarnecki, Olaf;Hedtke, Boris;Grimm, Bernhard
通讯作者：
Grimm, Bernhard
影响因子：
3.5
作者：
Schaffrath, U;Freydl, E;Dudler, R
通讯作者：
Dudler, R