Cloning and expression of a small heat shock protein gene CaHSP24 from pepper under abiotic stress
Cloning and expression of a small heat shock protein gene CaHSP24 from pepper under abiotic stress
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非生物胁迫下辣椒热激蛋白小基因CaHSP24的克隆及表达
DOI：
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发表时间：
2011
影响因子：
--
通讯作者：
Chen, Rugang
中科院分区：
文献类型：
--
作者：
Zhu, Wei;Lu, Minghui;Gong, Zhenhui;Chen, Rugang
采用基于同源性的候选基因方法和快速扩增cDNA末端（RACE）技术获得了小分子热休克蛋白（sHSP）基因CaHSP 24的序列。该基因cDNA序列全长920 bp（GenBank：HM 132040），开放阅读框（ORF）为636 bp，编码211个氨基酸残基的蛋白质。系统进化树分析表明，CaHSP 24与其他植物线粒体sHSP相似，但与辣椒细胞质和叶绿体sHSP的亲缘关系较远。半定量逆转录聚合酶链反应（RT-PCR）结果显示，CaHSP 24在32°C时几乎检测不到，而在40°C时明显积累。该基因在热胁迫后0.5 h开始表达，1.5 h表达量达到高峰，耐热品种B35的表达量高于热敏感品种B6。该基因在盐胁迫、重金属胁迫、低温胁迫和氧化胁迫下也有微弱表达，且表达量明显低于热胁迫。细胞活力实验表明，CaHSP 24的异源表达可提高大肠杆菌在热胁迫下的活力。综上所述，CaHSP 24可能在热应激反应中发挥重要作用。关键词：辣椒，CaHSP 24，热胁迫，基因表达。
The sequence of a small heat shock protein (sHSP) gene CaHSP24 was obtained by homology-based candidate gene method and rapid amplification of cDNA ends (RACE). The cDNA sequence of this gene is 920 bp in size (GenBank: HM132040) and contains an open reading frame (ORF) of 636 bp, which was predicted to encode a protein with 211 amino acid residues. The phylogenetic tree showed that CaHSP24 was quite similar to mitochondrial sHSPs from other plants but was distantly related to sHSPs of pepper cytoplasm and chloroplast. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) showed that CaHSP24 was hardly detectable at 32°C, but accumulated markedly at 40°C. The gene was expressed 0.5 h after exposure to heat stress and the expression reached a peak in 1.5 h; the expression level in heat-resistant cultivar B35 was higher than that in heat-sensitive cultivar B6. The gene was also expressed weakly under salinity, heavy metal, low temperature and oxidative stresses; the expression levels under these conditions were remarkably lower than those under heat stress. Cell viability experiments showed that the heterologous expression of CaHSP24 could enhance the viability of Escherichia coli under heat stress. To sum up, CaHSP24 may play an important role for response to heat stress condition. Key words : Pepper, CaHSP24, heat stress, gene expression.
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影响因子：
4.9
作者：
Liu, JA;Shono, M
通讯作者：
Shono, M
DOI：
10.1042/bj3110805
发表时间：
1995-11
期刊：
The Biochemical journal
影响因子：
--
作者：
C. Lenne;M. Block;J. Garin;R. Douce
通讯作者：
C. Lenne;M. Block;J. Garin;R. Douce
影响因子：
6.9
作者：
Giorno F;Wolters-Arts M;Grillo S;Scharf KD;Vriezen WH;Mariani C
通讯作者：
Mariani C
DOI：
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发表时间：
2001
期刊：
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影响因子：
--
作者：
Jian Liu;Shono Mariko
通讯作者：
Jian Liu;Shono Mariko
DOI：
10.1016/s0021-9258(18)53882-5
发表时间：
1993-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
U. Jakob;M. Gaestel;K. Engel;J. Buchner
通讯作者：
U. Jakob;M. Gaestel;K. Engel;J. Buchner