Developmental and heat stress-regulated expression of HsfA2 and small heat shock proteins in tomato anthers.
Developmental and heat stress-regulated expression of HsfA2 and small heat shock proteins in tomato anthers.
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DOI：
10.1093/jxb/erp316
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发表时间：
2010
影响因子：
6.9
通讯作者：
Mariani C
中科院分区：
文献类型：
--
作者：
Giorno F;Wolters-Arts M;Grillo S;Scharf KD;Vriezen WH;Mariani C
雄性生殖细胞对高温的高度敏感性可能是由于热应激反应不足所致。本文报道了热胁迫系统的两个重要成员HsfA2和Hsp17-CII在一个耐热番茄品种花药发育过程中的综合表达分析结果。利用半定量和实时荧光定量聚合酶链式反应技术对不同发育阶段的花药/花粉进行了转录分析。用原位RNA杂交定位信使，用免疫印迹分析监测蛋白质积累。根据基因和蛋白质表达谱的分析，HsfA2和Hsp17-CII在花药发育过程中受到精细调控，并在短期和长期热胁迫条件下进一步诱导。这些数据表明，HsfA2可能直接参与了番茄花药在热胁迫过程中保护机制的激活，从而可能有助于番茄在逆温条件下座果。
The high sensitivity of male reproductive cells to high temperatures may be due to an inadequate heat stress response. The results of a comprehensive expression analysis of HsfA2 and Hsp17-CII, two important members of the heat stress system, in the developing anthers of a heat-tolerant tomato genotype are reported here. A transcriptional analysis at different developmental anther/pollen stages was performed using semi-quantitative and real-time PCR. The messengers were localized using in situ RNA hybridization, and protein accumulation was monitored using immunoblot analysis. Based on the analysis of the gene and protein expression profiles, HsfA2 and Hsp17-CII are finely regulated during anther development and are further induced under both short and prolonged heat stress conditions. These data suggest that HsfA2 may be directly involved in the activation of protection mechanisms in the tomato anther during heat stress and, thereby, may contribute to tomato fruit set under adverse temperatures.
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影响因子：
6.9
作者：
Frank G;Pressman E;Ophir R;Althan L;Shaked R;Freedman M;Shen S;Firon N
通讯作者：
Firon N
DOI：
10.1002/dvg.1020140104
发表时间：
1993-01-01
期刊：
DEVELOPMENTAL GENETICS
影响因子：
--
作者：
ATKINSON, BG;RAIZADA, M;WALDEN, DB
通讯作者：
WALDEN, DB
影响因子：
--
作者：
Mascarenhas, JP;Crone, DF
通讯作者：
Crone, DF
影响因子：
7.2
作者：
Meiri, David;Breiman, Adina
通讯作者：
Breiman, Adina
影响因子：
7.4
作者：
Honys, D;Twell, D
通讯作者：
Twell, D