Quantification of cytokeratin 5 mRNA expression in the circulation of healthy human subjects and after lung transplantation.
Quantification of cytokeratin 5 mRNA expression in the circulation of healthy human subjects and after lung transplantation.
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DOI：
10.1371/journal.pone.0005925
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发表时间：
2009-06-16
期刊：
影响因子：
3.7
通讯作者：
Gomperts BN
中科院分区：
文献类型：
--
作者：
Nickerson DW;Presson AP;Weigt SS;Gregson AL;Belperio JA;Gomperts BN
循环上皮祖细胞对于气管移植小鼠模型中气道上皮的修复非常重要。因此，我们假设循环上皮祖细胞也存在于正常人受试者中，并且可能对肺损伤后气道的修复很重要。由于肺移植与肺损伤相关，肺损伤在早期是严重的，在感染和排斥反应期间会加重，我们假设循环上皮祖细胞水平可以预测肺移植后的临床结果。进行定量真实的时间PCR以确定外周血细胞角蛋白5的mRNA水平，细胞角蛋白5是循环上皮祖细胞的先前表征的标志物。在健康人受试者、肺移植受者移植后即刻和此后连续、以及心脏移植受者中评价细胞角蛋白5水平。所有检查的正常人受试者在其血沉棕黄层中表达细胞角蛋白5，其量不受年龄或性别的显著影响。与健康人受试者（p = 3.1 × 10 − 13）和心脏移植受者相比，肺移植患者的细胞角蛋白5 mRNA表达水平显著降低。 肺移植受者循环细胞角蛋白5 mRNA水平的改善与肺功能的恢复之间存在中度负相关性（通过FEV 1值的改善来衡量）（rho =-0.39）。 细胞角蛋白5 mRNA水平，循环上皮祖细胞的代理标记物，与肺移植受者的疾病状态呈负相关。因此，它可以作为肺移植患者和潜在的其他气道损伤患者的临床结果的生物标志物。
Circulating epithelial progenitor cells are important for repair of the airway epithelium in a mouse model of tracheal transplantation. We therefore hypothesized that circulating epithelial progenitor cells would also be present in normal human subjects and could be important for repair of the airway after lung injury. As lung transplantation is associated with lung injury, which is severe early on and exacerbated during episodes of infection and rejection, we hypothesized that circulating epithelial progenitor cell levels could predict clinical outcome following lung transplantation. Quantitative Real Time PCR was performed to determine peripheral blood mRNA levels of cytokeratin 5, a previously characterized marker of circulating epithelial progenitor cells. Cytokeratin 5 levels were evaluated in healthy human subjects, in lung transplant recipients immediately post-transplant and serially thereafter, and in heart transplant recipients. All normal human subjects examined expressed cytokeratin 5 in their buffy coat in amounts that were not significantly influenced by age or gender. There was a profound, statistically significant decrease in cytokeratin 5 mRNA expression levels in lung transplant patients compared to healthy human subjects (p = 3.1×10−13) and to heart transplant recipients. There was a moderate negative correlation between improved circulating cytokeratin 5 mRNA levels in lung transplant recipients with recovering lung function, as measured by improved FEV1 values (rho = −0.39). Levels of cytokeratin 5 mRNA, a proxy marker for circulating epithelial progenitor cells, inversely correlated with disease status in lung transplant recipients. It may therefore serve as a biomarker of the clinical outcome of lung transplant patients and potentially other patients with airway injury.
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DOI：
10.1165/rcmb.2005-0175rc
发表时间：
2005-10-01
影响因子：
6.4
作者：
Kotton, DN;Fabian, AJ;Mulligan, RC
通讯作者：
Mulligan, RC
影响因子：
6.2
作者：
Mattson, J;Jansson, M;Hassan, M
通讯作者：
Hassan, M
影响因子：
6
作者：
Hong, KU;Reynolds, SD;Stripp, BR
通讯作者：
Stripp, BR
影响因子：
6
作者：
Kleeberger, W;Versmold, A;Kreipe, H
通讯作者：
Kreipe, H
DOI：
10.1165/ajrcmb.24.6.4217
发表时间：
2001-06-01
影响因子：
6.4
作者：
Borthwick, DW;Shahbazian, M;Randell, SH
通讯作者：
Randell, SH