Dynamic expression of cadherins regulates vocal development in a songbird.
Dynamic expression of cadherins regulates vocal development in a songbird.
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DOI：
10.1371/journal.pone.0025272
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Okanoya K
中科院分区：
文献类型：
--
作者：
Matsunaga E;Suzuki K;Kato S;Kurotani T;Kobayashi K;Okanoya K
由于与人类相似，鸣禽在幼年阶段通过模仿学习发声，因此它们经常被用作研究人类语言学习机制的模型动物。大量的解剖学和生理学研究表明，鸣禽的大脑中有一个称为“歌唱系统”的神经网络，专门用于声音的学习和产生。然而，目前还不清楚是什么分子机制调控它们的发声发育。已经表明II型钙粘蛋白参与突触的形成和功能。在此之前，我们发现，II型钙粘蛋白的表达切换在强大的核arcopallium从钙粘蛋白-7-阳性钙粘蛋白-6B-阳性的阶段，从感觉到感觉运动学习阶段的鸣禽，孟加拉雀。此外，在体外培养的大鼠海马神经元的分析表明，钙粘蛋白-6B增强和钙粘蛋白-7通过调节树突棘形态抑制的微小兴奋性突触后电流的频率。为了探索钙粘蛋白在发声发育中的作用，我们用慢病毒载体对钙粘蛋白功能进行了体内行为分析。钙粘蛋白-7在幼年和成年阶段的过度表达导致发声的严重缺陷。在这两种情况下，通常在成年孟加拉雀歌曲中看到的和声特别受到影响。我们的研究结果表明，钙粘蛋白控制发声，特别是谐波的声音，可能是通过调节RA核的神经元形态。从感觉到感觉运动学习阶段的钙粘蛋白表达的转换似乎增强了发声能力，以尝试和错误的方式进行各种类型的发声，这对感觉运动学习至关重要。
Since, similarly to humans, songbirds learn their vocalization through imitation during their juvenile stage, they have often been used as model animals to study the mechanisms of human verbal learning. Numerous anatomical and physiological studies have suggested that songbirds have a neural network called ‘song system’ specialized for vocal learning and production in their brain. However, it still remains unknown what molecular mechanisms regulate their vocal development. It has been suggested that type-II cadherins are involved in synapse formation and function. Previously, we found that type-II cadherin expressions are switched in the robust nucleus of arcopallium from cadherin-7-positive to cadherin-6B-positive during the phase from sensory to sensorimotor learning stage in a songbird, the Bengalese finch. Furthermore, in vitro analysis using cultured rat hippocampal neurons revealed that cadherin-6B enhanced and cadherin-7 suppressed the frequency of miniature excitatory postsynaptic currents via regulating dendritic spine morphology. To explore the role of cadherins in vocal development, we performed an in vivo behavioral analysis of cadherin function with lentiviral vectors. Overexpression of cadherin-7 in the juvenile and the adult stages resulted in severe defects in vocal production. In both cases, harmonic sounds typically seen in the adult Bengalese finch songs were particularly affected. Our results suggest that cadherins control vocal production, particularly harmonic sounds, probably by modulating neuronal morphology of the RA nucleus. It appears that the switching of cadherin expressions from sensory to sensorimotor learning stage enhances vocal production ability to make various types of vocalization that is essential for sensorimotor learning in a trial and error manner.
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影响因子：
2.5
作者：
NOTTEBOHM, F;KELLEY, DB;PATON, JA
通讯作者：
PATON, JA
影响因子：
2.5
作者：
NOTTEBOHM, F;STOKES, TM;LEONARD, CM
通讯作者：
LEONARD, CM
影响因子：
25
作者：
London, Sarah E.;Clayton, David F.
通讯作者：
Clayton, David F.
DOI：
10.1196/annals.1298.026
发表时间：
2004-01-01
期刊：
BEHAVIORAL NEUROBIOLOGY OF BIRDSONG
影响因子：
--
作者：
Okanoya, K
通讯作者：
Okanoya, K
影响因子：
0.9
作者：
Honda, E;Okanoya, K
通讯作者：
Okanoya, K