Heme oxygenase-1 expression protects melanocytes from stress-induced cell death: implications for vitiligo.
Heme oxygenase-1 expression protects melanocytes from stress-induced cell death: implications for vitiligo.
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DOI：
10.1111/j.1600-0625.2010.01232.x
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发表时间：
2011-06
影响因子：
3.6
通讯作者：
Le Poole IC
中科院分区：
文献类型：
--
作者：
Elassiuty YE;Klarquist J;Speiser J;Yousef RM;El Refaee AA;Hunter NS;Shaker OG;Gundeti M;Nieuweboer-Krobotova L;Le Poole IC
为了研究血红素加氧酶在白癜风脱色和复色过程中对黑素细胞应激诱导的细胞死亡的保护作用，研究了紫外线或漂白剂4-TBP对培养的对照和患者黑素细胞以及正常皮肤组织中异构体1和2的表达。同样，在PUVA治疗前后，白癜风患者皮肤中的血红素氧合酶的表达也被跟踪观察。采用单、双免疫组织化学染色，结合光镜、共聚焦显微镜分析和Western blotting。黑素细胞对紫外线和4-TBP的反应上调了血红素加氧酶1的表达，而降低了血红素加氧酶2的表达。在紫外线处理的外植体培养中，在成功接受PUVA治疗的患者的皮肤中，以及从白癜风非皮损皮肤培养的黑素细胞中，也观察到了诱导型血红素氧合酶1的上调。随后克隆了编码血红素加氧酶的基因，以研究两种基因产物对细胞活力的影响，结果表明，在四甲基偶氮唑盐实验中，HO-1而不是HO-2的过度表达可以保护细胞免受应激诱导的细胞死亡。黑素细胞HO-1的表达可能与紫外线治疗白癜风的疗效有关。
To study protection of melanocytes from stress-induced cell death by heme oxygenases during depigmentation and repigmentation in vitiligo, expression of isoforms 1 and 2 was studied in cultured control and patient melanocytes and normal skin explants exposed to UV or bleaching agent 4-TBP. Similarly, expression of heme oxygenases was followed in skin from vitiligo patients before and after PUVA treatment. Single and double immunostainings were used in combination with light and confocal microscopic analysis and Western blotting. Melanocyte expression of heme oxygenase 1 is upregulated, whereas heme oxygenase 2 is reduced in response to UV and 4-TBP. Upregulation of inducible heme oxygenase 1 was also observed in UV-treated explant cultures, in skin of successfully PUVA-treated patients and in melanocytes cultured from vitiligo non-lesional skin. Heme oxygenase encoding genes were subsequently cloned to study consequences of either gene product on cell viability, demonstrating that HO-1 but not HO-2 overexpression offers protection from stress-induced cell death in MTT assays. HO-1 expression by melanocytes may contribute to beneficial effects of UV treatment for vitiligo patients.
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影响因子：
4.8
作者：
Kokot, Agatha;Metze, Dieter;Boehm, Markus
通讯作者：
Boehm, Markus
影响因子：
3.6
作者：
Listopad, Joanna;Asadullah, Khusru;Doecke, Wolf-Dietrich
通讯作者：
Doecke, Wolf-Dietrich
DOI：
10.1111/j.1600-0781.2006.00229.x
发表时间：
2006-08-01
影响因子：
2.6
作者：
El-Mofty, Medhat;Mostafa, Wedad;Fawzy, Marwa
通讯作者：
Fawzy, Marwa
影响因子：
3.3
作者：
Marrot, L;Belaïdi, JP;Meunier, JR
通讯作者：
Meunier, JR
影响因子：
7.4
作者：
Briganti, Stefania;Wlaschek, Meinhard;Scharffetter-Kochanek, Karin
通讯作者：
Scharffetter-Kochanek, Karin