MOLECULAR ANALYSIS OF THE ALLELES OF ALCOHOL DEHYDROGENASE ALONG A CLINE IN DROSOPHILA MELANOGASTER. I. MAINE, NORTH CAROLINA, AND FLORIDA
MOLECULAR ANALYSIS OF THE ALLELES OF ALCOHOL DEHYDROGENASE ALONG A CLINE IN DROSOPHILA MELANOGASTER. I. MAINE, NORTH CAROLINA, AND FLORIDA
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果蝇乙醇脱氢酶等位基因沿线的分子分析。
DOI：
10.1111/j.1558-5646.1989.tb04235.x
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发表时间：
1989
期刊：
影响因子：
3.3
通讯作者：
N. Miyashita
中科院分区：
文献类型：
--
作者：
G. M. Simmons;M. Kreitman;W. F. Quattlebaum;N. Miyashita
自然种群的临床变异通常被认为是自然选择的结果。然而，对于许多谱系，存在似是而非的中性解释，认为群体结构的某些方面在亚群体之间保持着特定性状的差异。我们使用限制性定位技术研究了黑腹果蝇群体细分和选择对乙醇脱氢酶（Adh）位点等位酶多态性维持的贡献。用7种酶对270种果蝇的基因组dna进行消化，发现在含有Adh结构位点的2.7千碱基区域中，有15-20%的核苷酸替换和几乎所有的插入/删除变异。对美国东海岸三个人口的大样本分析提供了在最北部人口中奠基者效应的证据。尽管样本之间存在种群分化的迹象，但两个种群之间的相似性表明种群之间的迁移是广泛的，并加强了自然选择在维持种群变化中起作用的论点。
Clinal variation in natural populations is often assumed to be due to the operation of natural selection. However, for many clines there exist plausible neutralist explanations which suggest that aspects of population structure maintain differences among subpopulations for particular traits. We used a restriction‐mapping technique to investigate the contributions of population subdivision and selection to the maintenance of the allozyme polymorphism at the alcohol dehydrogenase (Adh) locus of Drosophila melanogaster. Digestions of genomic DNAs from 270 lines of flies by seven enzymes reveal 15–20% of all possible nucleotide substitutions and virtually all of the insertion/deletion variation in a 2.7‐kilobase region containing the Adh structural locus. Analysis of large samples from each of three populations along the east coast of the United States provides evidence of founder effects in the most northerly population. Although there are signs of population differentiation among the samples, similarities between two of the populations indicate that migration among populations is extensive and strengthen the argument that natural selection plays a role in maintaining the cline.
DOI：
10.1073/pnas.83.10.3562
发表时间：
1986-05-01
影响因子：
11.1
作者：
KREITMAN, M;AGUADE, M
通讯作者：
AGUADE, M