Radial shockwave treatment promotes human mesenchymal stem cell self-renewal and enhances cartilage healing.
Radial shockwave treatment promotes human mesenchymal stem cell self-renewal and enhances cartilage healing.
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径向冲击波治疗促进人类间充质干细胞自我更新并增强软骨愈合。
DOI：
10.1186/s13287-018-0805-5
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发表时间：
2018-03-09
影响因子：
7.5
通讯作者：
Zhu H
中科院分区：
文献类型：
--
作者：
Zhang H;Li ZL;Yang F;Zhang Q;Su XZ;Li J;Zhang N;Liu CH;Mao N;Zhu H
冲击波和间充质干细胞（MSC）已被广泛接受为许多骨科应用的有用工具。然而，冲击波对MSC的调节作用仍然存在争议。在这项研究中，我们探讨了径向冲击波对人骨髓间充质干细胞的影响，使用浮动模型在体外和评估这些细胞对软骨缺损的愈合效果在体内使用兔模型。在体外培养的MSC，收获，重悬，并在浮动系统中用不同剂量的径向冲击波处理。通过生长动力学和细胞计数试剂盒-8（CCK-8）测定来评估细胞增殖。另外，通过荧光激活细胞分选术分析细胞周期和凋亡活性。为了探索MSC的“干性”，进行细胞集落形成试验和多分化测定。我们还使用透射电子显微镜检查了MSC的亚细胞结构，并通过病理分析检查了这些细胞对软骨缺损的愈合作用。生长动力学和CCK-8测定结果表明，径向冲击波处理显著促进MSC增殖。增强的细胞生长也反映了在S期细胞的数量增加，并在G 0/G1期细胞的数量减少，在冲击波处理的MSC中被逮捕。出乎意料的是，冲击波导致MSC凋亡率略有增加。此外，径向冲击波促进骨髓间充质干细胞的自我复制活性。透射电子显微镜显示，MSC的代谢活化的冲击波治疗。此外，径向冲击波有利于MSC成骨分化，但抑制成脂活性。最重要的是，经径向冲击波预处理的MSC在体内对软骨缺损表现出增强的愈合效果。与对照组相比，冲击波处理的MSCs复合生物支架显著改善兔膝关节损伤的组织学评分。在本研究中，我们发现径向冲击波在体外显著促进了MSC的增殖和自我更新，并安全地加速了体内软骨修复过程，表明了良好的临床结果。本文的在线版本（10.1186/s13287-018-0805-5）包含补充材料，可供授权用户使用。
Shockwaves and mesenchymal stem cells (MSCs) have been widely accepted as useful tools for many orthopedic applications. However, the modulatory effects of shockwaves on MSCs remain controversial. In this study, we explored the influence of radial shockwaves on human bone marrow MSCs using a floating model in vitro and evaluated the healing effects of these cells on cartilage defects in vivo using a rabbit model. MSCs were cultured in vitro, harvested, resuspended, and treated with various doses of radial shockwaves in a floating system. Cell proliferation was evaluated by growth kinetics and Cell Counting Kit-8 (CCK-8) assay. In addition, the cell cycle and apoptotic activity were analyzed by fluorescence activated cell sorting. To explore the “stemness” of MSCs, cell colony-forming tests and multidifferentiation assays were performed. We also examined the MSC subcellular structure using transmission electron microscopy and examined the healing effects of these cells on cartilage defects by pathological analyses. The results of growth kinetics and CCK-8 assays showed that radial shockwave treatment significantly promoted MSC proliferation. Enhanced cell growth was also reflected by an increase in the numbers of cells in the S phase and a decrease in the numbers of cells arrested in the G0/G1 phase in shockwave-treated MSCs. Unexpectedly, shockwaves caused a slight increase in MSC apoptosis rates. Furthermore, radial shockwaves promoted self-replicating activity of MSCs. Transmission electron microscopy revealed that MSCs were metabolically activated by shockwave treatment. In addition, radial shockwaves favored MSC osteogenic differentiation but inhibited adipogenic activity. Most importantly, MSCs pretreated by radial shockwaves exhibited an enhanced healing effect on cartilage defects in vivo. Compared with control groups, shockwave-treated MSCs combined with bio-scaffolds significantly improved histological scores of injured rabbit knees. In the present study, we found that radial shockwaves significantly promoted the proliferation and self-renewal of MSCs in vitro and safely accelerated the cartilage repair process in vivo, indicating favorable clinical outcomes. The online version of this article (10.1186/s13287-018-0805-5) contains supplementary material, which is available to authorized users.
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影响因子：
1.2
作者：
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通讯作者：
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