Tissue response, macrophage phenotype, and intrinsic calcification induced by cardiovascular biomaterials: Can clinical regenerative potential be predicted in a rat subcutaneous implant model?
Tissue response, macrophage phenotype, and intrinsic calcification induced by cardiovascular biomaterials: Can clinical regenerative potential be predicted in a rat subcutaneous implant model?
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DOI：
10.1002/jbm.a.37280
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Badylak SF
中科院分区：
文献类型：
--
作者：
Cramer M;Chang J;Li H;Serrero A;El-Kurdi M;Cox M;Schoen FJ;Badylak SF
宿主对植入生物材料的免疫反应，特别是浸润性巨噬细胞的表型，是生物相容性和下游重塑结果的关键决定因素。本研究采用皮下大鼠模型，比较由戊二醛固定牛心包（GF-BP）组成的生物支架（心脏瓣膜置换术的标准护理）与基于聚碳酸酯的超分子聚合物支架（ePC-UPy）、膀胱细胞外基质（uvm - ecm）和聚丙烯网（PP）组成的生物支架的组织反应，包括巨噬细胞表型、重塑电位和钙化倾向。ePC-UPy和UBM-ECM材料在2天内诱导单个核细胞浸润整个支架厚度，在14天内诱导新生血管形成。GF-BP和PP诱导了促炎（m1样）和抗炎（m2样）巨噬细胞的平衡，而UBM-ECM和ePC-UPy在所有时间点都支持显性的m2样巨噬细胞表型。与GF-BP相比，在180天的研究期间，ePC-UPy对钙化的易感程度明显降低。UBM-ECM诱导了典型的以m2样巨噬细胞为主的建设性重构反应，PP引起了典型的以m1样巨噬细胞为主的异物反应。本研究结果强调了巨噬细胞和宿主对具有不同物理和化学性质的生物材料的不同重塑反应，并提示大鼠皮下植入模型可用于预测心血管生物材料的体内生物相容性和再生潜力，用于临床应用。
The host immune response to an implanted biomaterial, particularly the phenotype of infiltrating macrophages, is a key determinant of biocompatibility and downstream remodeling outcome. The present study used a subcutaneous rat model to compare the tissue response, including macrophage phenotype, remodeling potential and calcification propensity of a biologic scaffold composed of glutaraldehyde-fixed bovine pericardium (GF-BP), the standard of care for heart valve replacement, with those of an electrospun polycarbonate-based supramolecular polymer scaffold (ePC-UPy), urinary bladder extracellular matrix (UBM-ECM), and a polypropylene mesh (PP). The ePC-UPy and UBM-ECM materials induced infiltration of mononuclear cells throughout the thickness of the scaffold within 2 days and neovascularization at 14 days. GF-BP and PP elicited a balance of pro-inflammatory (M1-like) and anti-inflammatory (M2-like) macrophages, while UBM-ECM and ePC-UPy supported a dominant M2-like macrophage phenotype at all timepoints. Relative to GF-BP, ePC-UPy was markedly less susceptible to calcification for the 180 day duration of the study. UBM-ECM induced an archetypical constructive remodeling response dominated by M2-like macrophages and the PP caused a typical foreign body reaction dominated by M1-like macrophages. The results of this study highlight the divergent macrophage and host remodeling response to biomaterials with distinct physical and chemical properties and suggest that the rat subcutaneous implantation model can be used to predict in vivo biocompatibility and regenerative potential for clinical application of cardiovascular biomaterials.
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影响因子：
4.6
作者：
Johnston, Douglas R.;Soltesz, Edward G.;Vakil, Nakul;Rajeswaran, Jeevanantham;Roselli, Eric E.;Sabik, Joseph F., III;Smedira, Nicholas G.;Svensson, Lars G.;Lytle, Bruce W.;Blackstone, Eugene H.
通讯作者：
Blackstone, Eugene H.
DOI：
10.1002/jbm.a.36013
发表时间：
2017-05
期刊：
Journal of biomedical materials research. Part A
影响因子：
--
作者：
Hachim D;Wang N;Lopresti ST;Stahl EC;Umeda YU;Rege RD;Carey ST;Mani D;Brown BN
通讯作者：
Brown BN
影响因子：
2.2
作者：
Amigo, Natalia;Martin Riganti, Juan;Nieponice, Alejandro
通讯作者：
Nieponice, Alejandro
影响因子：
9.7
作者：
Brown, Bryan N.;Londono, Ricardo;Tottey, Stephen;Zhang, Li;Kukla, Kathryn A.;Wolf, Matthew T.;Daly, Kerry A.;Reing, Janet E.;Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.
DOI：
10.1016/j.jtcvs.2017.12.046
发表时间：
2018-06-01
影响因子：
6
作者：
Bennink, Ger;Torii, Sho;Virmani, Renu
通讯作者：
Virmani, Renu