The molecular phenotype of kisspeptin neurons in the medial amygdala of female mice.
The molecular phenotype of kisspeptin neurons in the medial amygdala of female mice.
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DOI：
10.3389/fendo.2023.1093592
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
生殖是通过下丘脑-垂体-性腺(HPG)轴调节的，主要是通过下丘脑中Kispeptin神经元的作用。重要的是，Kiss1神经元已经在其他大脑区域被发现，包括杏仁内侧核(Mea)。虽然mea参与调节生殖生理和行为的各个方面，以及非生殖过程，但mea Kiss1神经元的功能在很大程度上是未知的。此外，除了雌激素的刺激外，我们对MeA Kiss1神经元是如何调控的知之甚少。使用RiboTag小鼠模型和RNA-seq，我们研究了mea Kiss1神经元的分子图谱，以确定在雌性小鼠mea Kiss1神经元中共表达的转录本，以及这些转录本是否受到雌激素(E2)处理的调节。RNA-seq鉴定了13,800个在雌性Mea Kiss1神经元中共表达的基因转录本，包括与生殖、新陈代谢和其他神经内分泌功能有关的神经肽和受体的基因。在Mea Kiss1神经元共表达的13,800个基因中，只有45个基因由于E2处理而表现出显著不同的表达水平。E2处理后，Kiss1、Gal和OXTR等基因转录增加，而ESR1和Cyp26b1等基因转录减少。应用双重RNAScope和免疫组织化学方法验证mea Kiss1与CCK和Cartpt的共表达。这些结果首次建立了Mea Kiss1神经元活跃表达的基因图谱，其中包括受E2调控的基因亚集，这为进一步研究Mea Kiss1神经元的调控和功能提供了有用的基础。
Reproduction is regulated through the hypothalamic-pituitary-gonadal (HPG) axis, largely via the action of kisspeptin neurons in the hypothalamus. Importantly, Kiss1 neurons have been identified in other brain regions, including the medial amygdala (MeA). Though the MeA is implicated in regulating aspects of both reproductive physiology and behavior, as well as non-reproductive processes, the functional roles of MeA Kiss1 neurons are largely unknown. Additionally, besides their stimulation by estrogen, little is known about how MeA Kiss1 neurons are regulated. Using a RiboTag mouse model in conjunction with RNA-seq, we examined the molecular profile of MeA Kiss1 neurons to identify transcripts that are co-expressed in MeA Kiss1 neurons of female mice and whether these transcripts are modulated by estradiol (E2) treatment. RNA-seq identified >13,800 gene transcripts co-expressed in female MeA Kiss1 neurons, including genes for neuropeptides and receptors implicated in reproduction, metabolism, and other neuroendocrine functions. Of the >13,800 genes co-expressed in MeA Kiss1 neurons, only 45 genes demonstrated significantly different expression levels due to E2 treatment. Gene transcripts such as Kiss1, Gal, and Oxtr increased in response to E2 treatment, while fewer transcripts, such as Esr1 and Cyp26b1, were downregulated by E2. Dual RNAscope and immunohistochemistry was performed to validate co-expression of MeA Kiss1 with Cck and Cartpt. These results are the first to establish a profile of genes actively expressed by MeA Kiss1 neurons, including a subset of genes regulated by E2, which provides a useful foundation for future investigations into the regulation and function of MeA Kiss1 neurons.
影响因子：
3.3
作者：
Maras, P. M.;Petrulis, A.
通讯作者：
Petrulis, A.
影响因子：
2.6
作者：
Huang, Lijuan;Guo, Jianlin;Xie, Yan;Zhou, Yunyu;Wu, Xiaofei;Li, Hui;Peng, Yun;Li, Ningdong
通讯作者：
Li, Ningdong