II. Stromal and Epithelial Cells from Rat Ventral Prostate during Androgen Deprivation and Estrogen Treatment — Regulation of Transcription
II. Stromal and Epithelial Cells from Rat Ventral Prostate during Androgen Deprivation and Estrogen Treatment — Regulation of Transcription
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II. 雄激素剥夺和雌激素治疗期间大鼠腹侧前列腺的基质细胞和上皮细胞 - 转录调节
DOI：
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
G. Aumüller
中科院分区：
文献类型：
--
作者：
M. Bacher;U. Rausch;Goebel Hw;B. Polzar;H. Mannherz;G. Aumüller
为了确定前列腺组织的功能能力，类固醇激素受体，生长因子，癌基因和特定的酶的表达在RNA水平上研究在不同的激素条件下（雄激素剥夺，雌激素治疗）大鼠腹侧前列腺（RVP）的分离的基质和上皮细胞。Slot blot和北方印迹分析分离的RNA导致特征性的变化：在对照组中，雄激素受体（AR）mRNA在完整前列腺的上皮中高，但在间质中低。在去势后的最初24小时内，其水平在上皮中增加，而在去势一周后，基质中仅略有增加。雌激素处理上皮和间质中的AR信号没有改变。相反，雌激素受体（ER）mRNA，主要在间质和上皮细胞非常低，减少去势后24小时内在间质和上皮细胞，并在一周后消失。雌激素治疗后，ER信号在间质中显著增加。碱性成纤维细胞生长因子（bFGF）和转化生长因子β（TGF-β）的mRNA仅在基质中发现。两者在对照组中均较低，并且在24小时内以不同的方式对去势和雌激素治疗作出反应。雌激素治疗的动物中bFGF增加，而TGF-β通过去势诱导。去势后不久（2小时）v-fos表达增加，并在6小时后达到最大值，但在12小时后在上皮中检测不到。（250字处删节）
To identify the functional capacities of prostatic tissue, the expression of steroid hormone receptors, growth factors, oncogenes and particular enzymes was studied at the RNA level in isolated stromal and epithelial cells of rat ventral prostate (RVP) under different hormonal conditions (androgen deprivation, estrogen treatment). Slot blot and Northern blot analyses of isolated RNA resulted in characteristic changes: In the control prostate, androgen receptor (AR) mRNA was high in epithelium of intact prostate, but low in stroma. Its level was increased after castration in the epithelium during the initial 24 hours, whereas an only slight increase occurred in stroma after one week castration. The AR signal was not altered by estradiol treatment in epithelium and stroma. Conversely, the estrogen receptor (ER) mRNA, predominant in stroma and very low in epithelium, decreased after castration in stroma and epithelium within 24 hours and was absent one week later. After estrogen treatment the ER signal increased considerably in stroma. mRNA of both basic fibroblast growth factor (bFGF) and transforming growth factor beta (TGF-beta) were exclusively found in stroma. Both were low in controls and responded in a different way to castration and estrogen treatment within 24 hours. bFGF was increased in estrogen-treatment animals, while TGF-beta was induced by castration. Shortly after castration (2 hours) v-fos expression increased and reached a maximum after 6 hours, but was no more detectable after 12 hours in epithelium.(ABSTRACT TRUNCATED AT 250 WORDS)
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DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
White,BA;Bancroft,FC
通讯作者：
Bancroft,FC
影响因子：
--
作者：
QUARMBY, VE;YARBROUGH, WG;WILSON, EM
通讯作者：
WILSON, EM
恒河猴前列腺中的雌激素和孕激素受体以及芳香酶活性。
影响因子：
4.8
作者：
West,NB;Roselli,CE;Resko,JA;Greene,GL;Brenner,RM
通讯作者：
Brenner,RM
影响因子：
56.9
作者：
DOOLITTLE, RF;HUNKAPILLER, MW;ANTONIADES, HN
通讯作者：
ANTONIADES, HN
前列腺上皮细胞系的密度依赖性极化分泌。
DOI：
10.1002/pros.2990200104
发表时间：
1992
期刊：
The Prostate
影响因子：
--
作者：
Djakiew,D;Pflug,B;Delsite,R;Lynch,JH;Onoda,M
通讯作者：
Onoda,M