Alteration of N-ras gene mutation after relapse in acute lymphoblastic leukemia.
Alteration of N-ras gene mutation after relapse in acute lymphoblastic leukemia.
复制标题
急性淋巴细胞白血病复发后 N-ras 基因突变的改变。
作者：
N. Terada;J. Miyoshi;K. Kawa;H. Sasai;S. Orita;K. Yumura‐Yagi;J. Hara;A. Fujinami;T. Kakunaga
采用聚合酶链式反应（PCR）和寡核苷酸杂交方法研究了N-ras在儿童急性淋巴细胞白血病（dALL）中的激活情况。N-ras基因点突变频率不高（2 / 15），并对1例阳性复发病例进行了详细分析。虽然在发病和复发时均检测到N-ras基因激活，但突变位点不同。发病时，Gly （GGT）在第12密码子上转变为Ser (AGT)，复发时，Gly （GGT）在同一密码子上转变为Asp （GAT）。此外，复发时的DNA在两个独立的受体细胞系上显示出明显高于发病时的DNA转化活性。免疫球蛋白（Ig）重链和t细胞受体(TCR) γ链基因相同的细胞表面表型和相同的重排模式表明，发病和复发的白血病细胞来源于同一前体细胞。因此，本病例支持ras激活不是启动白血病发生的事件，但可能参与白血病进展的概念。
We investigated N-ras activation in childhood acute lymphoblastic leukemia (dALL) by the polymerase chain reaction (PCR) and the oligonucleotide hybridization method. The frequency of point-mutation of the N-ras gene was not high (2 of 15), and one positive case who relapsed was analyzed in detail. Although N-ras gene activation was detected at both onset and relapse, the mutation sites were different. At onset, Gly (GGT) was changed to Ser (AGT) at codon 12, and at relapse, Gly (GGT) to Asp (GAT) was observed at the same codon. In addition, the DNA at relapse showed a remarkably higher transforming activity than the DNA at onset on two independent recipient cell lines. The identical cell surface phenotype and the same rearrangement patterns of both the immunoglobulin (Ig) heavy chain and T-cell receptor (TCR) gamma chain genes indicated that the leukemic cells at onset and those at relapse were derived from the same precursor cell. Therefore, this case supports the concept that ras activation is not the event initiating leukemogenesis, but may be involved in leukemic progression.
ras 癌基因对 EBV 感染的人 B 淋巴母细胞的转化和类浆细胞分化。
DOI：
10.1126/science.2536954
发表时间：
1989
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Seremetis,S;Inghirami,G;Ferrero,D;Newcomb,EW;Knowles,DM;Dotto,GP;Dalla-Favera,R
通讯作者：
Dalla-Favera,R
DOI：
10.1073/pnas.85.23.9268
发表时间：
1988-12-01
影响因子：
11.1
作者：
NERI, A;KNOWLES, DM;DALLAFAVERA, R
通讯作者：
DALLAFAVERA, R