Microinjection of activated phosphatidylinositol-3 kinase induces process outgrowth in rat PC12 cells through the Rac-JNK signal transduction pathway.
Microinjection of activated phosphatidylinositol-3 kinase induces process outgrowth in rat PC12 cells through the Rac-JNK signal transduction pathway.
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显微注射活化的磷脂酰肌醇-3 激酶通过 Rac-JNK 信号转导途径诱导大鼠 PC12 细胞的生长。
DOI：
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发表时间：
1998
影响因子：
4
通讯作者：
Yasuhisa Fukui
中科院分区：
文献类型：
--
作者：
Yoshihiro Kita;Koutarou D. Kimura;Koutarou D. Kimura;Michimoto Kobayashi;S. Ihara;Kozo Kaibuchi;Shinya Kuroda;M. Ui;H. Iba;Hiroaki Konishi;U. Kikkawa;Satoshi Nagata;Yasuhisa Fukui
我们先前已经证明，持续的磷脂酰肌醇(PI)-3激酶活性是神经生长因子(NGF)诱导PC12细胞突起生长所必需的。显微注射PI-3活性突变体可诱导突起形成，提示PI-3激酶确实参与了轴突的生长。然而，突起似乎是不完整的突起，因为它们的F-肌动蛋白和GAP43抗原的组织很差。含突起细胞的微管网络增强，突起形成被秋水仙素抑制，提示微管在PI-3激酶下游突起形成中起重要作用。Rac和Sek1/SAPK的显性-负性突变体可以抑制这些细胞的反应，而显性-负性突变体RAS和MAPK(MEK)抑制剂PD98059不能抑制这些反应，这表明参与这一过程形成的不是Ras-MAP激酶途径，而是Rac-Jun氨基末端激酶(JNK)途径。
We have previously shown that sustained phosphatidylinositol (PI)-3 kinase activity is necessary for neurite outgrowth of PC12 cells induced by nerve growth factor (NGF). Microinjection of a constitutively active mutant of PI-3 kinase induced process formation suggesting that PI-3 kinase is indeed involved in the neurite outgrowth. However, the processes appeared to be incomplete neurites as they had very poor organization of F-actin and GAP43 antigen. The microtubule network was enhanced in the process-bearing cells and process formation was inhibited by colchicine suggesting that microtubules play an important role in process formation downstream of PI-3 kinase. These cell responses were inhibited by dominant-negative mutants of Rac and Sek1/SAPK but not by a dominant-negative mutant Ras and PD98059, a MAP kinase kinase (MEK) inhibitor, suggesting that not the Ras-MAP kinase pathway but the Rac-Jun N-terminal kinase (JNK) pathway is involved in process formation.
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磷脂酰肌醇 3-激酶产生的多磷酸肌醇是大鼠肝脏和牛脑中磷脂酶 C 的不良底物。
DOI：
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发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Serunian,LA;Haber,MT;Fukui,T;Kim,JW;Rhee,SG;Lowenstein,JM;Cantley,LC
通讯作者：
Cantley,LC
DOI：
10.1016/s0021-9258(18)41950-3
发表时间：
1992-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Soltoff;S. Rabin;L. Cantley;D. Kaplan
通讯作者：
S. Soltoff;S. Rabin;L. Cantley;D. Kaplan
3-磷酸磷脂酰肌醇存在于正常和转化的成纤维细胞中，并且能抵抗牛脑磷脂酶 C II 的水解。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lips,DL;Majerus,PW;Gorga,FR;Young,AT;Benjamin,TL
通讯作者：
Benjamin,TL
I 型磷脂酰肌醇激酶活性与多种癌基因产物相关。
DOI：
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发表时间：
1989
期刊：
Oncogene research
影响因子：
--
作者：
Fukui,Y;Kornbluth,S;Jong,SM;Wang,LH;Hanafusa,H
通讯作者：
Hanafusa,H
影响因子：
2
作者：
Valente, Andre;Huang, Kuo-Hua;Engert, Florian
通讯作者：
Engert, Florian