Correlation between Methylation of Human Papillomavirus-16 L1 Gene and Cervical Carcinoma in Uyghur Women
Correlation between Methylation of Human Papillomavirus-16 L1 Gene and Cervical Carcinoma in Uyghur Women
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维吾尔族女性人乳头瘤病毒16 L1基因甲基化与宫颈癌的相关性
DOI：
10.1159/000444585
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发表时间：
2016-03
影响因子：
2.1
通讯作者：
Lu Ping
中科院分区：
文献类型：
--
作者：
Niyazi Mayineur;Sui Shuang;Zhu Kaichun;Wang Lin;Jiao Zhen;Lu Ping
工作背景：本研究旨在探讨人乳头瘤病毒（HPV）-16 L1基因CpG甲基化与维吾尔族妇女宫颈癌持续感染及发生的关系。研究方法：在4,364名维吾尔族妇女中，从145名（3.3%）HPV-16单一感染病例，分为5组：一过性感染（n = 32），持续性感染宫颈上皮内瘤变（CIN）1级（CIN 1，n = 21）、CIN 2 -3（n = 33）和浸润性宫颈癌（n = 38）组。采用焦磷酸测序法检测HPV-16 L1基因甲基化水平，应用受试者工作特征曲线（ROC曲线）评价HPV-16 L1基因甲基化水平对宫颈上皮内瘤样病变（CIN 2+）的预测和诊断价值。结果如下：随着疾病的进展，在13个CpG位点检测到甲基化增加，高甲基化水平与CIN 2+的风险相关。最强相关位点为6650（OR 9.89，95% CI 3.57-27.44）。区分CIN 2+和<CIN 2的每个CpG位点甲基化的ROC曲线下面积（AUC）范围为0.756至0.862，并且最大AUC在位置6650（AUC 0.862，95%CI 0.803-0.920）。CpG位点6389、6457、6581、6650、6796和7034的高甲基化水平与HPV持续感染的风险增加有关，其中CpG位点6389的甲基化水平与HPV持续感染的风险最高（OR 13.33，95%CI 3.95-28.08）。预测HPV持续感染的AUC范围为0.656-0.943，诊断价值最高的部位为6389（AUC 0.943，95%CI 0.884-1.000）。结论：提示HPV-16 L1区CpG位点甲基化对预测HPV持续感染和宫颈癌前病变具有重要价值。
Background: This study aims at determining the correlation between CpG methylation in human papillomavirus (HPV)-16 L1 and the persistent infections and development of cervical carcinoma in Uyghur women. Methods: Among the 4,364 Uyghur women, specimens were collected from 145 (3.3%) HPV-16 single infected cases, which were divided into 5 groups: transient infection (n = 32), persistent infection (n = 21, 12 months), cervical intraepithelial neoplasia (CIN) grade 1 (CIN1, n = 21), CIN2-3 (n = 33) and invasive cervical cancer (n = 38) groups. Methylation level in HPV-16 L1 was quantified by pyrosequencing, and values in the prediction and diagnosis of CIN2+ lesions were evaluated with receiver operating characteristic curves. Results: With the progression of the disease, increased methylation was detected at 13 CpG sites, and a high methylation level was associated with the risk of CIN2+. The strongest related site was 6650 (OR 9.89, 95% CI 3.57-27.44). The area under ROC curve (AUC) of methylation at each CpG site to differentiate between CIN2+ and <CIN2 ranged from 0.756 to 0.862, and the greatest AUC was at position 6650 (AUC 0.862, 95% CI 0.803-0.920). A high methylation level at CpG site 6389, 6457, 6581, 6650, 6796 and 7034 was connected with increased risk of HPV-persistent infection, and the strongest associated CpG site was 6389 (OR 13.33, 95% CI 3.95-28.08). The AUC in the prediction of HPV persistent infection was in the range of 0.656-0.943 and the site with the highest diagnostic value was 6389 (AUC 0.943, 95% CI 0.884-1.000). Conclusions: These results indicate that the methylation of CpG sites in HPV-16 L1 has a great value in contributing to the prediction of HPV-persistent infection and cervical precancerous progression.
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影响因子：
1.8
作者：
Melsheimer, P;Kaul, S;Bastert, G
通讯作者：
Bastert, G
DOI：
10.3760/cma.j.issn.0253-9624.2010.05.009
发表时间：
2010-05
影响因子：
--
作者：
F. Zhao;Shangying Hu;Siwei Zhang;Wanqing Chen;Y. Qiao
通讯作者：
F. Zhao;Shangying Hu;Siwei Zhang;Wanqing Chen;Y. Qiao
首次性交年龄过早和怀孕过早是发展中国家宫颈癌的危险因素
DOI：
10.1016/s1090-798x(09)79109-x
发表时间：
2009
期刊：
Yearbook of Obstetrics, Gynecology and Women's Health
影响因子：
--
作者：
L. Shulman
通讯作者：
L. Shulman
影响因子：
3.7
作者：
Kalantari, Mina;Garcia-Carranca, Alejandro;Dalia Morales-Vazquez, Claudia;Zuna, Rosemary;Perez Montiel, Delia;Calleja-Macias, Itzel E.;Johansson, Bo;Andersson, Sonia;Bernard, Hans-Ulrich
通讯作者：
Bernard, Hans-Ulrich
影响因子：
6.4
作者：
Lorincz, Attila T.;Brentnall, Adam R.;Vasiljevic, Natasa;Scibior-Bentkowska, Dorota;Castanon, Alejandra;Fiander, Alison;Powell, Ned;Tristram, Amanda;Cuzick, Jack;Sasieni, Peter
通讯作者：
Sasieni, Peter