Developmental Expression of the β‐Amyloid Precursor Protein and Heat‐Shock Protein 70 in the Cerebral Hemisphere Region of the Rat Brain
Developmental Expression of the β‐Amyloid Precursor Protein and Heat‐Shock Protein 70 in the Cerebral Hemisphere Region of the Rat Brain
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β-淀粉样前体蛋白和热休克蛋白 70 在大鼠大脑半球区域的发育表达
DOI：
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发表时间：
2002
影响因子：
5.2
通讯作者：
J. Nurnberger
中科院分区：
文献类型：
--
作者：
D. K. Lahiri;C. Nall;D. Chen;M. Zaphiriou;C. Morgan;J. Nurnberger
摘要：阿尔茨海默病（Alzheimer's disease，AD）是以β淀粉样蛋白（amyloid β protein，Aβ）在脑内沉积为特征的疾病。Aβ（约14kDa）来源于一个大的（约110kDa）β淀粉样前体蛋白（APP）家族，其是整合的膜糖蛋白。虽然最近有人提出了AD和酗酒之间的联系，但这种关系尚未在分子水平上进行探索。我们的假设是APP在大脑发育中起作用，APP水平异常可能与AD和酗酒相关的痴呆症有关。我们比较了乙醇未处理的酒精偏好（P）和酒精非偏好（NP）大鼠之间的总APP水平。我们还研究了APP水平可以以年龄依赖性方式调节年轻大鼠的可能性。我们研究了两种蛋白质在不同发育时期大鼠大脑半球区域的分布。使用了由以下不同年龄大鼠组成的6个组：7、14、21、36、43和78（出生后）天。使用mAb22C11对来自脑不同区域的细胞提取物进行蛋白质免疫印迹。我们的结果表明，7日龄P大鼠脑提取物中高分子量APP条带的水平高于其他测试样本，并且不同年龄P大鼠脑提取物中APP水平的分布比NP大鼠更不均匀。这些初步结果表明，APP可能在大鼠大脑的早期发育中发挥重要作用，而酒精偏好特性可能会影响发育中大脑的APP处理。
Abstract: Alzheimer's disease (AD) is characterized by depositions of the amyloid β protein (Aβ) in the brain in the form of extracellular plaques and cerebrovascular amyloid. Aβ (∼4 kDa) is derived from a family of large (∼110 kDa) β‐amyloid precursor proteins (APP), which are integral membrane glycoproteins. Although a connection between AD and alcoholism has recently been suggested, this relationship has not been explored at the molecular level. Our hypothesis is that APP has a role in brain development and that abnormal APP levels may be involved in dementia associated with AD and alcoholism. We compared the profile of total APP levels between ethanol naïve alcohol‐preferring (P) and alcohol‐nonpreferring (NP) rats. We also investigated the possibility that APP levels can be regulated in an age‐dependent manner in young rats. We studied the distribution of two proteins in the cerebral hemisphere region of the rat brain at various developmental periods. Six groups composed of the following different ages of rats were used: 7, 14, 21, 36, 43, and 78 (postnatal) days. Cell extracts from different regions of the brain were subjected to Western immunoblotting using mAb22C11. Our results suggest that levels of high‐molecular‐weight APP bands were greater in brain extracts from 7‐day‐old P rats than in other samples tested, and that the distribution of APP levels was more uneven in brain extracts from different ages of P than from NP rats. These initial results suggest that APP may play an important role in the early development of the rat brain and the alcohol‐preferring trait may influence APP processing in the developing brain.
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DOI：
10.1073/pnas.90.24.12045
发表时间：
1993-12-15
影响因子：
11.1
作者：
DAIGLE, I;LI, C
通讯作者：
LI, C
小鼠 β-淀粉样蛋白前体蛋白 (APP) 普遍存在的交叉反应同源物的表达。
DOI：
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发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Slunt,HH;Thinakaran,G;VonKoch,C;Lo,AC;Tanzi,RE;Sisodia,SS
通讯作者：
Sisodia,SS
通过偏好乙醇和不偏好乙醇的大鼠系进行胃内自我输注乙醇。
DOI：
10.1126/science.6539502
发表时间：
1984
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Waller,MB;McBride,WJ;Gatto,GJ;Lumeng,L;Li,TK
通讯作者：
Li,TK
根据影响饮酒的因素选择啮齿动物品系。
DOI：
--
发表时间：
1987
期刊：
Alcohol and alcoholism (Oxford, Oxfordshire). Supplement.
影响因子：
--
作者：
Li,TK;Lumeng,L;McBride,WJ;Murphy,JM
通讯作者：
Murphy,JM
DOI：
10.1111/j.1530-0277.2000.tb01993.x
发表时间：
2000-12-01
影响因子：
3.2
作者：
Lewohl, JM;Wang, L;Harris, RA
通讯作者：
Harris, RA