Establishment of a murine pancreatic cancer pain model and microarray analysis of pain‑associated genes in the spinal cord dorsal horn.
Establishment of a murine pancreatic cancer pain model and microarray analysis of pain‑associated genes in the spinal cord dorsal horn.
复制标题
分子医学报告
DOI：
10.3892/mmr.2017.7173
复制
发表时间：
2017-10
影响因子：
3.4
通讯作者：
Lu Z
中科院分区：
文献类型：
--
作者：
Wang L;Xu H;Ge Y;Zhu H;Yu D;Yu W;Lu Z
胰腺癌疼痛的机制有新的证据。在建立胰腺癌的正交各向异性移植模型后，进行微阵列分析以鉴定脊髓中疼痛相关基因表达水平的变化。通过将SW 1990细胞植入雌性BALB/c-nu小鼠的胰腺中来建立胰腺癌诱导的疼痛的小鼠模型。观察大鼠原位移植后存活率和体重变化。采用大体解剖技术和苏木精-伊红染色对胰腺肿瘤组织进行分析。还进行了多种行为测试以评估疼痛相关反应。此外，使用来自具有或不具有可观察到的疼痛的小鼠的样品，进行微阵列分析以确定脊髓背角中的基因表达谱。在手术后的最初3周期间，患有胰腺癌的小鼠的存活率很高，尽管体重逐渐下降。大体解剖技术表明，手术后肿瘤大小显著增加，并且该结果通过小鼠模型的胰腺组织中的实体瘤肿块得到证实。通过测量腹部皮肤的机械阈值、弓背行为和内脏反应，还在胰腺癌模型中检查了可观察到的疼痛行为反应。还鉴定了脊髓背角中准确反映疾病分子病理学进展的10个疼痛特异性相关基因的谱。总之，本研究开发了一种新的动物模型，胰腺癌疼痛的BALB/c-nu小鼠，类似于人类胰腺癌疼痛，疼痛相关基因的表达在脊髓背角已被描绘。本研究的结果可能进一步了解介导胰腺癌疼痛的分子机制。
There is emerging evidence on the mechanisms of pancreatic cancer pain. Following the establishment of an orthotropic transplantation model of pancreatic cancer, microarray analysis was performed to identify changes in the expression levels of pain-associated genes in the spinal cord. A mouse model of pancreatic cancer-induced pain was established by implanting SW 1990 cells into the pancreases of female BALB/c-nu mice. The survival rate and body weight were measured following orthotropic transplantation. Gross anatomical techniques and hematoxylin and eosin staining were used to analyze the pancreatic tumor tissue. Multiple behavioral tests were also performed to assess pain-associated responses. Additionally, using samples from mice with or without observable pain, microarray analysis was performed to determine the gene expression profiles in the spinal cord dorsal horn. The survival rate of mice with pancreatic cancer was high during the initial 3 weeks post-surgery, although the body weight decreased progressively. Gross anatomical techniques demonstrated that the tumor size increased significantly following the surgery, and this result was confirmed by solid tumor masses in the pancreatic tissues of the mouse model. Observable pain behavioral responses were also examined in the pancreatic cancer model by measuring the mechanical threshold of the abdominal skin, hunching behavior and visceromotor responses. The profiles of 10 pain specific-associated genes in the spinal cord dorsal horn that accurately reflect the molecular pathological progression of disease were also identified. In conclusion, the present study has developed a novel animal model of pancreatic cancer pain in BALB/c-nu mice that resembles human pancreatic cancer pain, and the expression of pain-associated genes in the spinal cord dorsal horn has been profiled. The results of the present study may further the understanding of the molecular mechanisms that mediate pancreatic cancer pain.
登录
查看更多内容
影响因子：
4
作者：
Fillingim, Roger B.;Bruehl, Stephen;Dworkin, Robert H.;Dworkin, Samuel F.;Loeser, John D.;Turk, Dennis C.;Widerstrom-Noga, Eva;Arnold, Lesley;Bennett, Robert;Edwards, Robert R.;Freeman, Roy;Gewandter, Jennifer;Hertz, Sharon;Hochberg, Marc;Krane, Elliot;Mantyh, Patrick W.;Markman, John;Neogi, Tuhina;Ohrbach, Richard;Paice, Judith A.;Porreca, Frank;Rappaport, Bob A.;Smith, Shannon M.;Smith, Thomas J.;Sullivan, Mark D.;Verne, G. Nicholas;Wasan, Ajay D.;Wesselmann, Ursula
通讯作者：
Wesselmann, Ursula
DOI：
10.1158/1078-0432.ccr-15-3163
发表时间：
2016-09-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Magrangeas F;Kuiper R;Avet-Loiseau H;Gouraud W;Guérin-Charbonnel C;Ferrer L;Aussem A;Elghazel H;Suhard J;Sakissian H;Attal M;C Munshi N;Sonneveld P;Dumontet C;Moreau P;van Duin M;Campion L;Minvielle S
通讯作者：
Minvielle S
影响因子：
50.5
作者：
Cherny, NI
通讯作者：
Cherny, NI
影响因子：
4.7
作者：
Forman, Arthur D
通讯作者：
Forman, Arthur D
影响因子：
5.6
作者：
Li, Wei;Zhang, Yu;Zhang, Mengyuan
通讯作者：
Zhang, Mengyuan