Biochemical characterization of pulmonary washings of patients with alveolar proteinosis, interstitial pneumonitis and alveolar cell carcinoma.
Biochemical characterization of pulmonary washings of patients with alveolar proteinosis, interstitial pneumonitis and alveolar cell carcinoma.
复制标题
肺泡蛋白沉积症、间质性肺炎和肺泡细胞癌患者肺部洗出液的生化特征。
DOI：
10.1620/tjem.139.245
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
T. Akino
中科院分区：
文献类型：
--
作者：
T. Onodera;M. Nakamura;T. Sato;T. Akino
我们研究了七名肺泡蛋白沉积症患者的十五份肺洗液、两名间质性肺炎患者的两份肺洗液和两名肺泡细胞癌患者的两份肺洗液的生化成分。通过短暂离心将肺部洗液分离成上清液和沉淀物部分。分析结果显示，与其他患者的肺洗液相比，肺泡蛋白沉积症患者的肺洗液含有更多的蛋白质和脂质，以及更高比例的磷脂。对于肺泡蛋白沉积症，沉淀部分，即水不溶性物质，含有脂质作为主要成分，其中大部分是二棕榈酰磷脂酰胆碱。沉积物中的蛋白质含量较少，但经SDS-凝胶电泳测定，主要由分子量为62,000、36,000、28,000和15,000的蛋白质组成。另一方面，上清液部分，即水溶性物质，主要由血清蛋白组成，脂质含量低于沉淀部分中的脂质含量。这些分析结果支持这样的观点：肺泡中正常存在的物质在肺泡蛋白沉积症中过度积累为肺泡填充物质。还指出，肺泡蛋白沉积症患者的肺灌洗液与其他疾病患者的肺灌洗液的脂质谱存在显着差异，表明肺灌洗液的生化成分往往反映潜在疾病的性质。根据这些发现，讨论了肺泡蛋白沉积症中发现的肺泡填充物质的原因。
We have studied the biochemical compositions of fifteen pulmonary washings from seven patients with alveolar proteinosis, and two washings from two patients with interstitial pneumonitis and two from two patients with alveolar cell carcinoma. The pulmonary washing was separated into the supernatant and precipitate fractions by a brief centrifugation. Analytical results revealed that the pulmonary washings from patients with alveolar proteinosis contained much more protein and lipids as well as a higher percentage of phospholipid than did the pulmonary washings from other patients. With regards to alveolar proteinosis, the precipitate fraction, i.e., water-insoluble material, contained lipids as the major component, the majority of which was dipalmitoyl phosphatidylcholine. Protein in the sedimental material was small in amount, but was composed of proteins mainly of molecular weights of 62,000, 36,000, 28,000 and 15,000 as measured by SDS-gel electrophoresis. On the other hand, the supernatant fraction, i.e., water-soluble material, was composed predominantly of serum proteins, with the lipid content being lower than those in the precipitate fraction. These analytical findings support the idea that materials normally existing in the alveoli are excessively accumulated as alveolar-filling materials in alveolar proteinosis. It was also noted that there were marked differences in the lipid profiles between pulmonary washings from patients with alveolar proteinosis, and those from patients with other diseases, indicating that the biochemical composition of pulmonary washings tends to reflect the nature of an underlying disease. From these findings, the cause of the alveolar-filling materials found in alveolar proteinosis was discussed.
影响因子：
6.5
作者：
W. R. Morrison;L. Smith
通讯作者：
W. R. Morrison;L. Smith