Reticuloendotheliosis virus and avian leukosis virus subgroup J synergistically increase the accumulation of exosomal miRNAs.
Reticuloendotheliosis virus and avian leukosis virus subgroup J synergistically increase the accumulation of exosomal miRNAs.
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网状内皮增生病毒和禽白血病病毒J亚型协同增加外泌体miRNA的积累
DOI：
10.1186/s12977-018-0427-0
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发表时间：
2018-07-03
期刊：
影响因子：
3.3
通讯作者：
Cheng Z
中科院分区：
文献类型：
--
作者：
Zhou D;Xue J;He S;Du X;Zhou J;Li C;Huang L;Nair V;Yao Y;Cheng Z
研究背景J亚群禽白血病病毒与网状内皮组织增生症病毒共感染可产生协同致病作用，增加死亡率。然而，外泌体的miRNA的作用的分子机制的协同感染的两个viruss.ResultsIn这项研究中，外泌体RNA从感染ALV-J，REV或两者在最佳协同感染时间CEF细胞进行了分析Illumina RNA深度测序。通过比较两种病毒（分别为单独感染的ALV-J和REV）的共感染，共鉴定出54种（23种上调和31种下调）和16种（7种上调和9种下调）miRNA。此外，通过qRT-PCR在外来体和CEF细胞中验证了五种关键miRNA，包括miR-184- 3 p、miR-146 a-3 p、miR-146 a-5 p、miR-3538和miR-155。GO注释和KEGG通路分析表明，差异表达的5种miRNA参与了病毒-载体相互作用、氧化磷酸化、能量代谢和细胞生长等过程。结论REV和ALV-J协同作用增加了外泌体miRNA的积累，揭示了ALV-J和REV协同作用的分子机制。
BackgroundCo-infection with avian leukosis virus subgroup J and reticuloendotheliosis virus induces synergistic pathogenic effects and increases mortality. However, the role of exosomal miRNAs in the molecular mechanism of the synergistic infection of the two viruses remains unknown.ResultsIn this study, exosomal RNAs from CEF cells infected with ALV-J, REV or both at the optimal synergistic infection time were analysed by Illumina RNA deep sequencing. A total of 54 (23 upregulated and 31 downregulated) and 16 (7 upregulated and 9 downregulated) miRNAs were identified by comparing co-infection with two viruses, single-infected ALV-J and REV, respectively. Moreover, five key miRNAs, including miR-184-3p, miR-146a-3p, miR-146a-5p, miR-3538 and miR-155, were validated in both exosomes and CEF cells by qRT-PCR. GO annotation and KEGG pathway analysis of the miRNA target genes showed that the five differentially expressed miRNAs participated in virus-vector interaction, oxidative phosphorylation, energy metabolism and cell growth.ConclusionsWe demonstrated that REV and ALV-J synergistically increased the accumulation of exosomal miRNAs, which sheds light on the synergistic molecular mechanism of ALV-J and REV.
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影响因子：
64.8
作者：
Baek, Daehyun;Villen, Judit;Shin, Chanseok;Camargo, Fernando D.;Gygi, Steven P.;Bartel, David P.
通讯作者：
Bartel, David P.
影响因子：
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3.3
作者：
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影响因子：
2.2
作者：
Lamparski, HG;Metha-Damani, A;Le Pecq, JB
通讯作者：
Le Pecq, JB