Evaluation of novel derivatisation reagents for the analysis of oxysterols.
Evaluation of novel derivatisation reagents for the analysis of oxysterols.
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DOI：
10.1016/j.bbrc.2014.01.173
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发表时间：
2014-04-11
影响因子：
3.1
通讯作者：
Griffiths, William J.
中科院分区：
文献类型：
--
作者：
Crick, Peter J.;Aponte, Jennifer;Bentley, T. William;Matthews, Ian;Wang, Yuqin;Griffiths, William J.
用于氧固醇LC-MS分析的新衍生试剂。基于吉拉德P的新试剂提供高离子电流和信息LC-MSn光谱。永久电荷对于有效的MSn碎裂是至关重要的。新试剂为同位素标记的掺入提供了更大的范围。氧化固醇是胆固醇的氧化形式，是胆汁酸和类固醇激素合成的中间体。它们也是核和G蛋白偶联受体的配体。生物系统中氧固醇的分析具有挑战性，因为它们的丰度低，加上它们缺乏强发色团和质谱（MS）中的差电离特性。我们以前使用酶辅助衍生甾醇分析（EADSA），以确定和定量生物样品中的氧化甾醇。该技术依赖于用吉拉德P试剂标记甾醇以引入带电荷的季铵基团。在这里，我们已经比较了几个修改后的Girard样试剂，并表明永久电荷是至关重要的有效MSn碎片。然而，我们发现，该试剂可以扩展到包括潜在的稳定同位素标记的网站，而不会损失性能。
New derivatisation reagents for LC–MS analysis of oxysterols. New reagents based on Girard P give high ion-currents and informative LC–MSn spectra. Permanent charge is vital for efficient MSn fragmentation. New reagents offer greater scope for incorporation of isotope labels. Oxysterols are oxidised forms of cholesterol that are intermediates in the synthesis of bile acids and steroid hormones. They are also ligands to nuclear and G protein-coupled receptors. Analysis of oxysterols in biological systems is challenging due to their low abundance coupled with their lack of a strong chromophore and poor ionisation characteristics in mass spectrometry (MS). We have previously used enzyme-assisted derivatisation for sterol analysis (EADSA) to identify and quantitate oxysterols in biological samples. This technique relies on tagging sterols with the Girard P reagent to introduce a charged quaternary ammonium group. Here, we have compared several modified Girard-like reagents and show that the permanent charge is vital for efficient MSn fragmentation. However, we find that the reagent can be extended to include sites for potential stable isotope labels without a loss of performance.
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影响因子：
7.4
作者：
Thompson, A;Schäfer, J;Hamon, C
通讯作者：
Hamon, C
DOI：
10.1073/pnas.96.1.266
发表时间：
1999-01-05
影响因子：
11.1
作者：
Janowski, BA;Grogan, MJ;Mangelsdorf, DJ
通讯作者：
Mangelsdorf, DJ
影响因子：
64.8
作者：
Liu, Changlu;Yang, Xia V.;Lovenberg, Timothy W.
通讯作者：
Lovenberg, Timothy W.
27-羟基胆固醇促进细胞自主，ER阳性乳腺癌的生长。
影响因子：
8.8
作者：
Wu Q;Ishikawa T;Sirianni R;Tang H;McDonald JG;Yuhanna IS;Thompson B;Girard L;Mineo C;Brekken RA;Umetani M;Euhus DM;Xie Y;Shaul PW
通讯作者：
Shaul PW
DOI：
10.1016/j.bbalip.2010.02.012
发表时间：
2010-08
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Wang Y;Kumar N;Crumbley C;Griffin PR;Burris TP
通讯作者：
Burris TP