Growth of human colorectal cancer SW1116 cells is inhibited by cytokine-induced killer cells.
Growth of human colorectal cancer SW1116 cells is inhibited by cytokine-induced killer cells.
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DOI：
10.1155/2011/621414
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发表时间：
2011
影响因子：
--
通讯作者：
Han W
中科院分区：
文献类型：
--
作者：
Wang Y;Dai H;Li H;Lv H;Wang T;Fu X;Han W
以前的报道表明，用苦参碱诱导的杀伤（CIK）细胞治疗可能有益于患有各种类型肿瘤的患者。本研究的目的是评价CIK细胞对大肠癌细胞株SW 1116的体内外抗肿瘤作用。CIK细胞常规地从健康人供体的外周血单核细胞产生，并且在培养14天后，CD 3 + CD 56+细胞的数量扩增超过1300倍。在效应器处：将SW 1116细胞皮下注射的实验小鼠随机分为4组：未治疗组、5-氟尿嘧啶（5-FU）治疗组、CIK连续治疗组（每天注射1次）和CIK间隔治疗组（每5天注射1次）。CIK细胞共腹部注射五次。与未处理组相比，CIK处理的异种移植物生长受到极大抑制，其抑制程度与5-FU处理的抑制程度几乎相同。我们证明了CIK治疗组的肿瘤异种移植物的坏死面积明显大于其他组。这些研究结果表明，基于CIK的免疫治疗可能是结直肠癌患者的有效选择。
Previous reports have suggested that treatment with cytokine-induced killer (CIK) cells may benefit patients with various types of tumor. The aim of this study was to evaluate the antitumor effects of CIK cells against the colorectal cancer line SW1116 in vitro and in vivo. CIK cells were generated routinely from peripheral blood mononuclear cells of healthy human donors, and the number of CD3+CD56+ cells was expanded more than 1300-fold after 14-day culture. At an effector : target cell ratio of 50 : 1, the percentage lysis of SW1116 cells reached 68% in the presence of CIK cells, Experimental mice injected with SW1116 cells subcutaneously were divided randomly into four groups: untreated, 5-fluorouracil (5-FU)-treated, CIK-consecutive treated (injected once/day) and CIK-interval treated (injected once every 5 days). CIK cells were injected abdominally five times in total. Compared with the untreated group, xenograft growth was inhibited greatly by CIK treatment, to nearly the same extent as with 5-FU treatment. We demonstrated that the necrotic area in the tumor xenograft was markedly larger in the CIK-treated groups than in the other groups. These findings suggest that CIK-based immunotherapy may represent an effective choice for patients with colorectal cancer.
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影响因子：
254.7
作者：
Jemal, Ahmedin;Siegel, Rebecca;Thun, Michael J.
通讯作者：
Thun, Michael J.
DOI：
10.1084/jem.174.1.139
发表时间：
1991-07-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Schmidt-Wolf IG;Negrin RS;Kiem HP;Blume KG;Weissman IL
通讯作者：
Weissman IL
影响因子：
3.4
作者：
Liu, Pengying;Chen, Longbang;Huang, Xiang
通讯作者：
Huang, Xiang
影响因子：
20.3
作者：
Baker, J;Verneris, MR;Negrin, RS
通讯作者：
Negrin, RS
影响因子：
64.8
作者：
KAGI, D;LEDERMANN, B;HENGARTNER, H
通讯作者：
HENGARTNER, H