Blood cell gene expression associated with cellular stress defense is modulated by antioxidant-rich food in a randomised controlled clinical trial of male smokers.
Blood cell gene expression associated with cellular stress defense is modulated by antioxidant-rich food in a randomised controlled clinical trial of male smokers.
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DOI：
10.1186/1741-7015-8-54
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发表时间：
2010-09-16
期刊：
影响因子：
9.3
通讯作者：
Blomhoff R
中科院分区：
文献类型：
--
作者：
Bøhn SK;Myhrstad MC;Thoresen M;Holden M;Karlsen A;Tunheim SH;Erlund I;Svendsen M;Seljeflot I;Moskaug JO;Duttaroy AK;Laake P;Arnesen H;Tonstad S;Collins A;Drevon CA;Blomhoff R
富含水果和蔬菜的植物性饮食可以预防多种与年龄相关的慢性疾病。然而，这种保护作用背后的机制尚未阐明。我们测试了这样的假设：摄入富含抗氧化剂的食物会影响人类血细胞中与细胞应激防御相关的基因组。试验注册号：NCT00520819 http://clinicaltrials.gov。在一项为期 8 周的饮食干预研究中，102 名健康男性吸烟者被随机分配到富含各种富含抗氧化剂的食物的饮食组、奇异果饮食组（每天在常规饮食中添加三个奇异果）或对照组。每组随机选择 10 名个体，获得血细胞基因表达谱。通过对使用 Affymetrix HG-U133-Plus 2.0 全基因组阵列获得的转录谱进行基因集富集分析 (GSEA) 的新应用，将饮食引起的基因表达变化与对照进行比较。与对照组相比，两个干预组的血细胞基因表达谱均发生变化。与对照组相比，两种饮食均显着上调了参与细胞应激防御调节（例如 DNA 修复、细胞凋亡和缺氧）的基因组（GSEA、FDR q 值 < 5%）。两种干预措施均上调了具有芳基碳氢化合物受体 (AhR) 和 AhR 核转位子 (AhR/ARNT) 共同调控基序的基因（FDR q 值 < 5%）。两组的血浆抗氧化生物标志物（多酚/类胡萝卜素）均有所增加。观察到的血细胞基因表达谱的变化表明，植物性饮食对人类健康的有益影响可能是通过优化防御过程来介导的。
Plant-based diets rich in fruit and vegetables can prevent development of several chronic age-related diseases. However, the mechanisms behind this protective effect are not elucidated. We have tested the hypothesis that intake of antioxidant-rich foods can affect groups of genes associated with cellular stress defence in human blood cells. Trial registration number: NCT00520819 http://clinicaltrials.gov. In an 8-week dietary intervention study, 102 healthy male smokers were randomised to either a diet rich in various antioxidant-rich foods, a kiwifruit diet (three kiwifruits/d added to the regular diet) or a control group. Blood cell gene expression profiles were obtained from 10 randomly selected individuals of each group. Diet-induced changes on gene expression were compared to controls using a novel application of the gene set enrichment analysis (GSEA) on transcription profiles obtained using Affymetrix HG-U133-Plus 2.0 whole genome arrays. Changes were observed in the blood cell gene expression profiles in both intervention groups when compared to the control group. Groups of genes involved in regulation of cellular stress defence, such as DNA repair, apoptosis and hypoxia, were significantly upregulated (GSEA, FDR q-values < 5%) by both diets compared to the control group. Genes with common regulatory motifs for aryl hydrocarbon receptor (AhR) and AhR nuclear translocator (AhR/ARNT) were upregulated by both interventions (FDR q-values < 5%). Plasma antioxidant biomarkers (polyphenols/carotenoids) increased in both groups. The observed changes in the blood cell gene expression profiles suggest that the beneficial effects of a plant-based diet on human health may be mediated through optimization of defence processes.
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