Preparing for the next Ebola outbreak: in-country genomic capacity in Africa.
Preparing for the next Ebola outbreak: in-country genomic capacity in Africa.
复制标题
为下一次埃博拉疫情爆发做准备：非洲的国内基因组能力。
DOI：
10.1016/s1473-3099(19)30173-2
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Happi AN
中科院分区：
文献类型：
--
作者：
Happi AN
2018年5月，埃博拉病毒病（EVD）在刚果的赤道省死灰复燃，这是一个重要的提醒，提醒人们很难预测下一次疫情将在何时何地发生。随后于2018年7月在刚果民主共和国爆发的EVD疫情影响了约1000人，该疫情是迄今为止EVD的最高病死率（62%）。1冲突、不安全、贫穷、不信任、卫生系统差和其他因素都与持续爆发有关。对2018年7月北基伍省埃博拉病毒病疫情的应对凸显了2013-16年西非埃博拉病毒病疫情期间吸取的经验教训的积极影响。这些经验教训包括病毒的实时基因组分析和在疫情应对期间使用不同的应对措施，2北方半球和南半球国家之间以及南半球国家之间的强有力合作，以及为应对疾病爆发而进行的研究能力建设投资，3以及世卫组织和其他卫生发展机构及时和坚定的应对措施。4在《柳叶刀传染病》中，Placide Mbala-Kingebeni及其同事2展示了国内基因组学能力如何被整合到刚果民主共和国北基伍省当前的EVD疫情应对中。作者报告了使用基因组学工具评估疫情期间部署的诊断和治疗等医疗对策。Mbala-Kingebeni及其同事使用实时基因组病毒测序证明，在北基伍省和伊图里省发现的病毒株与2018年5月在赤道省爆发的EVD疫情中发现的病毒株不同。作者使用Illumina Technologies（San Diego，CA，USA）开发的Nextera Flex方法进行病毒基因组富集，将血液样本采集和全病毒基因组序列生成之间的时间从72小时缩短至28小时。他们还在可供实地部署的一些聚合酶链反应检测的目标区域发现了少量错配和突变;然而，这些错配都不影响聚合酶链反应检测的性能。
The resurgence of Ebola virus disease (EVD) in Équateur province in the Democratic Republic of the Congo in May 2018, is an important reminder of the difficulties of predicting when and where the next outbreak will occur. The subsequent July 2018 outbreak of EVD in the Democratic Republic of the Congo has affected about 1000 people and the outbreak has the highest case fatality rate (62%) for EVD to date. 1 Conflict, insecurity, poverty, mistrust, poor health systems, and other factors have been implicated in sustaining the outbreak. The response to the July 2018 EVD outbreak in North Kivu Province has highlighted the positive impact of lessons learnt during the 2013–16 EVD outbreak in west Africa. These lessons included real-time genomic analysis of viruses and the use of different countermeasures during the outbreak response, 2 strong collaborations between countries of the northern hemisphere and southern hemisphere and between countries within the southern hemisphere and rooted investment for research capacity building to address disease outbreaks, 3 andtimely and committed responses from WHO and other health development agencies. 4In the Lancet Infectious Diseases, Placide Mbala-Kingebeni and colleagues2 demonstrated how in-country genomics capacity has been integrated in the current EVD outbreak response in North Kivu Province of the Democratic Republic of the Congo. The authors report the use of genomics tools for assessment of medical countermeasures such as diagnostics and therapeutics deployed during the outbreak. Mbala-Kingebeni and colleagues used real-time genomic viral sequencing to demonstrate that the virus strain identified in the North Kivu and Ituri Provinces is different from the strain identified in the previous May 2018 EVD outbreak in Équateur Province. The authors used the Nextera Flex method for viral genome enrichment, developed by Illumina Technologies (San Diego, CA, USA), to shorten the time between blood sample collection and generation of whole viral genome sequences from 72 to 28 h. They also identified a small number of mismatches and mutations in targeted areas of some PCR tests available for field deployment; however, none of these mismatches affected the performance of the PCR tests.
暴力事件导致埃博拉疫情蔓延至 1,000 例
影响因子：
64.8
作者：
A. Maxmen
通讯作者：
A. Maxmen
对 2018 年尼日利亚拉沙病毒激增的基因组分析
影响因子：
158.5
作者：
K. Siddle;Philomena J. Eromon;K. Barnes;Samar B. Mehta;J. Oguzie;I. Odia;S. Schaffner;S. Winnicki;R. Shah;J. Qu;S. Wohl;Patrick Brehio;C. Iruolagbe;J. Aiyepada;Eghosa Uyigue;Patience Akhilomen;G. Okonofua;Simon H. Ye;Tolulope A Kayode;Fehintola V Ajogbasile;J. Uwanibe;A. Gaye;Mambu Momoh;B. Chak;Dylan Kotliar;Amber Carter;A. Gladden;C. A. Freije;Omigie Omoregie;B. Osiemi;Ekene B Muoebonam;M. Airende;R. Enigbe;B. Ebo;Iguosadolo Nosamiefan;P. Oluniyi;M. Nekoui;E. Ogbaini;R. Garry;K. Andersen;Daniel J. Park;N. Yozwiak;G. Akpede;C. Ihekweazu;O. Tomori;S. Okogbenin;O. Folarin;P. Okokhere;B. MacInnis;Pardis C Sabeti;C. Happi
通讯作者：
C. Happi