Drosophila forkhead homologues are expressed in a lineage-restricted manner in human hematopoietic cells.
Drosophila forkhead homologues are expressed in a lineage-restricted manner in human hematopoietic cells.
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果蝇叉头同源物在人类造血细胞中以谱系限制的方式表达。
DOI：
10.1182/blood.v81.11.2854.2854
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发表时间：
1993
期刊：
影响因子：
20.3
通讯作者：
M. Klemsz
中科院分区：
文献类型：
--
作者：
R. Hromas;J. Moore;T. Johnston;C. Socha;M. Klemsz
叉头基因（FKH）调节果蝇的形态发生。它是一个新的转录激活因子家族的原型。部分简并寡核苷酸的两个保守的氨基酸序列的这个家庭被用来引发聚合酶链反应（PCR）扩增的HEL细胞cDNA。分离出两个独特的克隆，命名为H3和H8，它们含有与FKH的同源性。第三个新的克隆，5-3，通过使用H8作为探针的慢性髓性白血病cDNA文库的低严格筛选而分离。H3和5-3在限制性造血谱系中优先表达，而H8的表达是普遍存在的。Southern分析表明FKH 5-3在酵母中是保守的，这在组织特异性转录因子中是罕见的。H3和5-3克隆提供了FKH家族成员以组织限制性方式存在于人类中的证据。
The forkhead gene (FKH) regulates morphogenesis in Drosophila. It is the prototype of a new family of transcriptional activators. Partially degenerate oligonucleotides to two conserved amino acid sequences of this family were used to prime a polymerase chain reaction (PCR) amplification of HEL cell cDNA. Two unique clones, designated H3 and H8, were isolated that contained homologies to FKH. A third novel clone, 5-3, was isolated by low stringency screening of a chronic myelogenous leukemia cDNA library using H8 as a probe. H3 and 5-3 are preferentially expressed in restricted hematopoietic lineages, while the expression of H8 was ubiquitous. Southern analysis showed that FKH 5-3 is conserved through yeast, which is rare among tissue-specific transcription factors. The H3 and 5-3 clones provide evidence that FKH family members are present in a tissue-restricted manner in humans.
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影响因子：
4.4
作者：
LI, C;LUSIS, AJ;GAYNOR, R
通讯作者：
GAYNOR, R
影响因子：
10.5
作者：
LAI, E;PREZIOSO, VR;DARNELL, JE
通讯作者：
DARNELL, JE
DOI：
--
发表时间：
1984-02
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
P. Andrews;I. Damjanov;D. Simon;G. Banting;C. Carlin;N. Dracopoli;J. Fogh
通讯作者：
P. Andrews;I. Damjanov;D. Simon;G. Banting;C. Carlin;N. Dracopoli;J. Fogh
影响因子：
10.5
作者：
LAI, E;PREZIOSO, VR;DARNELL, JE
通讯作者：
DARNELL, JE
影响因子：
4.4
作者：
LI, C;LUSIS, AJ;GAYNOR, R
通讯作者：
GAYNOR, R