Protein analysis of the spermatophore reveals diverse compositions in both the ampulla and the spermatophylax in a bushcricket
Protein analysis of the spermatophore reveals diverse compositions in both the ampulla and the spermatophylax in a bushcricket
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精囊的蛋白质分析揭示了蟋蟀壶腹部和精囊的不同组成
DOI：
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
P. Jungblut
中科院分区：
文献类型：
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作者：
G. Lehmann;K. Lehmann;B. Neumann;A. Lehmann;C. Scheler;P. Jungblut
婚礼礼物是雄性交配的投资，在丛林蟋蟀中，它包括含有精子的壶腹和附着的精原细胞。精巢的功能是阻止雌性过早移除含有精子的壶腹，这是雌性的营养来源，也是影响雌性行为的化合物的来源，以增加雄性的进化适合度。将这些功能放在蛋白质组学的角度，我们用大分子双向凝胶电泳法结合纳米液相色谱-电喷雾电离质谱仪和从头测序技术分析了雄性大蟋蟀(Poecilimon Ampliatus(Brunner von Wtenwyl，1878))新婚礼物的蛋白质组成。我们以高分辨率分离蛋白质，并在精液(壶腹)和精叶中检测到大约600个蛋白质点和300个蛋白质点。蛋白质斑点只有一小部分重叠，而两个隔间的大部分不同。由于缺乏这种非模式昆虫的基因组和蛋白质序列数据，我们无法识别蛋白质。根据潜在的蛋白质成本和潜在功能，我们讨论了蛋白质的多样性以及它们的大小范围。
Nuptial gifts are male mating investments, which, in bushcrickets, comprise the sperm‐containing ampulla and the attached spermatophylax. The functions of the spermatophylax are to deter females from premature removal of the sperm‐containing ampulla, which is a nutrient resource for females, as well as a source of compounds that influence female behaviour to increase male evolutionary fitness. Placing these functions into a proteomic perspective, we analyze the protein composition of nuptial gifts from male Poecilimon ampliatus (Brunner von Wattenwyl, 1878) bushcrickets using large two‐dimensional gel electrophoresis coupled with nano‐liquid chromatography‐electrospray ionization mass spectrometry and de novo sequencing. We separate the proteins with high resolution and detect approximately 600 protein spots in the seminal fluid (ampulla) and 300 in the spermatophylax. There is only a small fraction of overlap in protein spots, whereas the majority differ between the two compartments. As a result of the lack of a sequenced genome and protein data for this non‐model insect, we are unable to identify the proteins. We discuss the diversity of proteins, as well as their size range, in light of potential protein costs and potential functions.
影响因子：
23.8
作者：
Avila FW;Sirot LK;LaFlamme BA;Rubinstein CD;Wolfner MF
通讯作者：
Wolfner MF
影响因子：
16.8
作者：
Elser JJ;Acquisti C;Kumar S
通讯作者：
Kumar S
影响因子：
7
作者：
Findlay, Geoffrey D.;MacCoss, Michael J.;Swanson, Willie J.
通讯作者：
Swanson, Willie J.