Transgenic Microalgae Expressing Double-Stranded RNA as Potential Feed Supplements for Controlling White Spot Syndrome in Shrimp Aquaculture.
Transgenic Microalgae Expressing Double-Stranded RNA as Potential Feed Supplements for Controlling White Spot Syndrome in Shrimp Aquaculture.
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DOI：
10.3390/microorganisms11081893
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发表时间：
2023-07-26
期刊：
影响因子：
4.5
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中科院分区：
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养殖鱼类和贝类的病毒感染是水产养殖业的一个主要问题。一种潜在的控制策略涉及通过口服特定双链RNA（dsRNA）来RNA干扰病毒基因表达。在以前的工作中，我们已经表明，重组dsRNA可以在可食用的衣藻的叶绿体中产生，并用于控制虾的疾病。在这里，我们报告了一个显着的改善，抗病毒dsRNA的生产和使用，以保护虾对白色斑点综合征病毒（WSSV）。开发了一种新的dsRNA合成策略，其使用内源rrnS启动子的两个会聚拷贝来驱动叶绿体中WSSV基因元件的两条链的高水平转录。定量RT-PCR结果表明，每升转基因酵母培养物中可产生约119 ng dsRNA。这表示相对于我们先前的报告，dsRNA增加了约10倍。在用病毒攻击之前，评估工程化的对虾在饲喂虾幼虫时防止WSSV感染的能力。相对于阴性对照（<10%存活率），给予补充有含有dsRNA的干虾的饲料的虾的存活率显著提高（约69%存活率）。研究结果表明，这种新的dsRNA生产平台可以作为一种低成本、低技术的水产养殖控制方法。
Viral infection of farmed fish and shellfish represents a major issue within the aquaculture industry. One potential control strategy involves RNA interference of viral gene expression through the oral delivery of specific double-stranded RNA (dsRNA). In previous work, we have shown that recombinant dsRNA can be produced in the chloroplast of the edible microalga Chlamydomonas reinhardtii and used to control disease in shrimp. Here, we report a significant improvement in antiviral dsRNA production and its use to protect shrimp against white spot syndrome virus (WSSV). A new strategy for dsRNA synthesis was developed that uses two convergent copies of the endogenous rrnS promoter to drive high-level transcription of both strands of the WSSV gene element in the chloroplast. Quantitative RT-PCR indicated that ~119 ng dsRNA was produced per liter of culture of the transgenic microalga. This represents an ~10-fold increase in dsRNA relative to our previous report. The engineered alga was assessed for its ability to prevent WSSV infection when fed to shrimp larvae prior to a challenge with the virus. The survival of shrimp given feed supplemented with dried alga containing the dsRNA was significantly enhanced (~69% survival) relative to a negative control (<10% survival). The findings suggest that this new dsRNA production platform could be employed as a low-cost, low-tech control method for aquaculture.
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影响因子：
5.6
作者：
Jackson HO;Taunt HN;Mordaka PM;Smith AG;Purton S
通讯作者：
Purton S
影响因子：
4.7
作者：
Bird, Jasmine E.;Marles-Wright, Jon;Giachino, Andrea
通讯作者：
Giachino, Andrea
DOI：
10.5281/zenodo.10894766
发表时间：
2013-01-01
期刊：
MalariaWorld journal
影响因子：
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作者：
Kumar, Anil;Wang, Songjie;Sayre, Richard T
通讯作者：
Sayre, Richard T
质体中双链 RNA 的长度依赖性积累影响科罗拉多马铃薯甲虫的 RNA 干扰效率。
影响因子：
6.9
作者：
He, Wanwan;Xu, Wenbo;Zhang, Jiang
通讯作者：
Zhang, Jiang
影响因子：
4.5
作者：
通讯作者：
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