Soybean GmMYB133 Inhibits Hypocotyl Elongation and Confers Salt Tolerance in Arabidopsis.
Soybean GmMYB133 Inhibits Hypocotyl Elongation and Confers Salt Tolerance in Arabidopsis.
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大豆 GmMYB133 抑制拟南芥下胚轴伸长并赋予耐盐性
DOI：
10.3389/fpls.2021.764074
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发表时间：
2021
影响因子：
5.6
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Shan B;Wang W;Cao J;Xia S;Li R;Bian S;Li X
REVEILLE（RVE）基因通常作为核心昼夜振荡器来调节植物的多种发育事件和胁迫反应。研究它们在作物中的作用，对于利用它们改良农艺性状具有重要意义。大豆是世界上最重要的农作物之一。然而，关于RVE在大豆中的功能作用的知识非常有限。在这项研究中，大豆基因GmMYB 133被证明是同源的RVE 8进化枝基因的拟南芥。GmMYB 133表现出非节律性但盐诱导的表达模式。与AtRVE 8一样，GmMYB 133在拟南芥中的过表达导致了发育缺陷，如短下胚轴和延迟开花。GmMYB 133抑制了AtPIF 4等7个光响应或生长素相关基因的转录，表明GmMYB 133可能对植物生长具有负调控作用。在盐胁迫下，GmMYB 133基因的过表达促进了拟南芥种子萌发和植株生长，叶绿素和丙二醛（MDA）含量分别增加和降低。GmMYB 133的过表达显著提高了4种盐胁迫相关正调控因子的表达，表明GmMYB 133可能具有耐盐性。进一步观察发现GmMYB 133过表达扰乱了AtPRR 5的生物钟节律，酵母单杂交实验表明GmMYB 133可以与AtPRR 5启动子结合。此外，检索到的ChIP-Seq数据显示，AtPRR 5可以直接靶向包括AtPIF 4在内的五个客户端。因此，提出了一个调控模块GmMYB 133-PRR 5-PIF 4来调控植物生长和盐胁迫耐受性。这些研究结果为进一步阐明GmMYB 133在大豆中的功能作用及其调控机制奠定了基础。
REVEILLE (RVE) genes generally act as core circadian oscillators to regulate multiple developmental events and stress responses in plants. It is of importance to document their roles in crops for utilizing them to improve agronomic traits. Soybean is one of the most important crops worldwide. However, the knowledge regarding the functional roles of RVEs is extremely limited in soybean. In this study, the soybean gene GmMYB133 was shown to be homologous to the RVE8 clade genes of Arabidopsis. GmMYB133 displayed a non-rhythmical but salt-inducible expression pattern. Like AtRVE8, overexpression of GmMYB133 in Arabidopsis led to developmental defects such as short hypocotyl and late flowering. Seven light-responsive or auxin-associated genes including AtPIF4 were transcriptionally depressed by GmMYB133, suggesting that GmMYB133 might negatively regulate plant growth. Noticeably, the overexpression of GmMYB133 in Arabidopsis promoted seed germination and plant growth under salt stress, and the contents of chlorophylls and malondialdehyde (MDA) were also enhanced and decreased, respectively. Consistently, the expressions of four positive regulators responsive to salt tolerance were remarkably elevated by GmMYB133 overexpression, indicating that GmMYB133 might confer salt stress tolerance. Further observation showed that GmMYB133 overexpression perturbed the clock rhythm of AtPRR5, and yeast one-hybrid assay indicated that GmMYB133 could bind to the AtPRR5 promoter. Moreover, the retrieved ChIP-Seq data showed that AtPRR5 could directly target five clients including AtPIF4. Thus, a regulatory module GmMYB133-PRR5-PIF4 was proposed to regulate plant growth and salt stress tolerance. These findings laid a foundation to further address the functional roles of GmMYB133 and its regulatory mechanisms in soybean.
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