Comparison and evaluation of biomechanical, electrical, and biological methods for assessment of damage to tissue collagen.
Comparison and evaluation of biomechanical, electrical, and biological methods for assessment of damage to tissue collagen.
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DOI：
10.1007/s10561-016-9560-y
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发表时间：
2016-09
影响因子：
1.5
通讯作者：
Yao, Hai
中科院分区：
文献类型：
--
作者：
Hepfer, R. Glenn;Brockbank, Kelvin G. M.;Chen, Zhen;Greene, Elizabeth D.;Campbell, Lia H.;Wright, Gregory J.;Linthurst-Jones, Alyce;Yao, Hai
关于评估用于保存或以其他方式处理（处理）软同种异体移植物组织的组织库方法，目前的测试可能不足以检测在处理之前、期间和之后造成的潜在损伤。使用受控参数，我们的目的是检查特定的生物力学，电学和生物学测试在检测胶原蛋白轻度损伤的灵敏度。新鲜的猪肺动脉瓣用胶原酶处理，并使用不同的时间孵育。对照组未接受孵育。所有瓣膜均冷冻保存并储存在-135 °C下，直至复温，以使用生物力学、渗透性和细胞活力试验进行评价。在经过0-15分钟胶原酶处理的心脏瓣膜之间，发现瓣叶极限应力（p=0.006）、渗透性（p=0.01）和活力（p≤0.02，4个不同培养天数）的统计学显著性时间依赖性变化（ANOVA）。然而，在处理和未处理瓣膜的拉伸模量之间没有发现统计学显著性（p=0.07）。此外，在处理时间内，极限应力和活力分别降低和增加的趋势有些不一致。这些结果表明，渗透性测试可以提供一个敏感的，定量的分析，以补充传统的生物力学和活力测试，在评估用于软组织同种异体移植物的处理方法，或当改变目前已验证的方法。多项测试评估还可以深入了解潜在组织损伤的机制，例如，如此处的情况，胶原蛋白含量减少和组织孔隙率增加。
In regard to evaluating tissue banking methods used to preserve or otherwise treat (process) soft allograft tissue, current tests may not be sufficiently sensitive to detect potential damage inflicted before, during, and after processing. Using controlled parameters, we aim to examine the sensitivity of specific biomechanical, electrical, and biological tests in detecting mild damage to collagen. Fresh porcine pulmonary heart valves were treated with an enzyme, collagenase, and incubated using various times. Controls received no incubation. All valves were cryopreserved and stored at −135°C until being rewarmed for evaluation using biomechanical, permeability, and cell viability tests. Statistically significant time dependent changes in leaflet ultimate stress, (p=0.006), permeability (p=0.01), and viability (p≤0.02, 4 different days of culture) were found between heart valves subjected to 0–15 minutes of collagenase treatment (ANOVA). However, no statistical significance was found between the tensile modulus of treated and untreated valves (p=0.07). Furthermore, the trends of decreasing and increasing ultimate stress and viability, respectively, were somewhat inconsistent across treatment times. These results suggest that permeability tests may offer a sensitive, quantitative assay to complement traditional biomechanical and viability tests in evaluating processing methods used for soft tissue allografts, or when making changes to current validated methods. Multiple test evaluation may also offer insight into the mechanism of potential tissue damage such as, as is the case here, reduced collagen content and increased tissue porosity.
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影响因子：
3.8
作者：
Gu, WY;Yao, H;Flagler, D
通讯作者：
Flagler, D
心脏瓣膜组织的玻璃化。
DOI：
10.1007/978-1-4939-2193-5_20
发表时间：
2015
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Brockbank KG;Chen Z;Greene ED;Campbell LH
通讯作者：
Campbell LH
影响因子：
2.7
作者：
Brockbank, Kelvin G. M.;Heacox, Albert E.;Schenke-Layland, Katja
通讯作者：
Schenke-Layland, Katja
影响因子：
10.8
作者：
HU, JF;GILMER, L;WOLFINBARGER, L
通讯作者：
WOLFINBARGER, L
影响因子：
18.2
作者：
Hinton RB;Yutzey KE
通讯作者：
Yutzey KE