Continuous Monitoring via Tethered Electroencephalography of Spontaneous Recurrent Seizures in Mice.
Continuous Monitoring via Tethered Electroencephalography of Spontaneous Recurrent Seizures in Mice.
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DOI：
10.3389/fnbeh.2017.00172
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发表时间：
2017
影响因子：
3
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Bin NR;Song H;Wu C;Lau M;Sugita S;Eubanks JH;Zhang L
我们在这里描述了一种简单、经济有效的装置，用于连续拴系脑电图 (EEG) 监测小鼠自发性复发性癫痫发作。我们使用小型低扭矩滑环作为脑电图换向器，将滑环安装到标准小鼠笼上，并将滑环的旋转线直接连接到动物的植入耳机。对笼子进行了改造，以便方便安装滑环并适应动物周围的活动。我们在成年 C57 黑小鼠中测试了该装置的海马脑电图记录。使用延长海马点火（≥95 每日刺激）诱导自发性复发性癫痫发作。对照动物接受类似的海马电极植入，但不给予刺激。每天 24 小时进行脑电图和网络摄像头联合监测，连续 5-9 天。在监测期间，动物自由移动并获取水和食物，并且笼内活动没有表现出明显的限制。类似发作的海马脑电图放电和并发惊厥行为是自发性复发性癫痫发作的特征，在大多数长期点燃的监测实验中得到了可靠的记录，但在对照动物中却没有记录到。然而，在连续录音≥5天后，一些动物的植入耳机上有1-2根旋转线断开。讨论了我们的记录设备的主要特征和主要局限性。
We describe here a simple, cost-effective apparatus for continuous tethered electroencephalographic (EEG) monitoring of spontaneous recurrent seizures in mice. We used a small, low torque slip ring as an EEG commutator, mounted the slip ring onto a standard mouse cage and connected rotary wires of the slip ring directly to animal's implanted headset. Modifications were made in the cage to allow for a convenient installation of the slip ring and accommodation of animal ambient activity. We tested the apparatus for hippocampal EEG recordings in adult C57 black mice. Spontaneous recurrent seizures were induced using extended hippocampal kindling (≥95 daily stimulation). Control animals underwent similar hippocampal electrode implantations but no stimulations were given. Combined EEG and webcam monitoring were performed for 24 h daily for 5–9 consecutive days. During the monitoring periods, the animals moved and accessed water and food freely and showed no apparent restriction in ambient cage activities. Ictal-like hippocampal EEG discharges and concurrent convulsive behaviors that are characteristics of spontaneous recurrent seizures were reliably recorded in a majority of the monitoring experiments in extendedly kindled but not in control animals. However, 1–2 rotary wires were disconnected from the implanted headset in some animals after continuous recordings for ≥5 days. The key features and main limitations of our recording apparatus are discussed.
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