Uropod elongation is a common final step in leukocyte extravasation through inflamed vessels.
Uropod elongation is a common final step in leukocyte extravasation through inflamed vessels.
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DOI：
10.1084/jem.20111426
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发表时间：
2012-07-02
期刊：
影响因子：
--
通讯作者：
Kim M
中科院分区：
文献类型：
--
作者：
Hyun YM;Sumagin R;Sarangi PP;Lomakina E;Overstreet MG;Baker CM;Fowell DJ;Waugh RE;Sarelius IH;Kim M
尾足伸长发生在白细胞外渗期间。免疫细胞进入外周非淋巴组织的有效运输是发挥其保护功能的关键。尽管我们对次级淋巴器官细胞迁移的理解取得了相当大的进展，但白细胞向炎症组织的实时募集并没有很好地表征。白细胞外渗的传统多步骤模式依赖于CD18整合素介导的事件，如内皮表面的快速阻滞和爬行以及通过内皮层的转运。在新开发的敲入小鼠中使用增强的三维荧光CD18融合蛋白检测，我们报告了外渗的白细胞（中性粒细胞、单核细胞和T细胞）表现出延迟的尾足脱离，并且在完全穿过内皮细胞之前变得非常细长。此外，这些细胞在收缩拉伸的尾足之前将CD18+微粒沉积在内皮下层。敲除小鼠和阻断抗体实验表明，尾足的伸长和微粒的形成是lfa -1介导的粘附和vla -3介导的细胞通过血管基底膜迁移的结果。这些发现表明，尾足延伸是白细胞外渗级联的最后一步，这可能对白细胞向炎症组织募集的精确调节很重要。
Uropod elongation occurs during leukocyte extravasation. The efficient trafficking of immune cells into peripheral nonlymphoid tissues is key to enact their protective functions. Despite considerable advances in our understanding of cell migration in secondary lymphoid organs, real-time leukocyte recruitment into inflamed tissues is not well characterized. The conventional multistep paradigm of leukocyte extravasation depends on CD18 integrin–mediated events such as rapid arrest and crawling on the surface of the endothelium and transmigration through the endothelial layer. Using enhanced three-dimensional detection of fluorescent CD18 fusion proteins in a newly developed knockin mouse, we report that extravasating leukocytes (neutrophils, monocytes, and T cells) show delayed uropod detachment and become extremely elongated before complete transmigration across the endothelium. Additionally, these cells deposit CD18+ microparticles at the subendothelial layer before retracting the stretched uropod. Experiments with knockout mice and blocking antibodies reveal that the uropod elongation and microparticle formation are the result of LFA-1–mediated adhesion and VLA-3–mediated cell migration through the vascular basement membrane. These findings suggest that uropod elongation is a final step in the leukocyte extravasation cascade, which may be important for precise regulation of leukocyte recruitment into inflamed tissues.
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中性粒细胞的迁移引导是通过高亲和力LFA-1和钙通量来机械转化的。
DOI：
10.4049/jimmunol.1004197
发表时间：
2011-07-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Dixit N;Yamayoshi I;Nazarian A;Simon SI
通讯作者：
Simon SI
影响因子：
20.3
作者：
Elphick, Gwendolyn F.;Sarangi, Pranita P.;Kim, Minsoo
通讯作者：
Kim, Minsoo
影响因子：
3.7
作者：
Fuhr, G;Richter, E;Hagedorn, R
通讯作者：
Hagedorn, R
影响因子：
6.4
作者：
Ammon, C;Meyer, SP;Kreutz, M
通讯作者：
Kreutz, M
影响因子：
5.3
作者：
Jung, S;Aliberti, J;Littman, DR
通讯作者：
Littman, DR