A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants.
A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants.
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DOI：
10.3390/v13050885
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发表时间：
2021-05-11
期刊：
影响因子：
--
通讯作者：
Lomonossoff GP
中科院分区：
文献类型：
--
作者：
Thuenemann EC;Byrne MJ;Peyret H;Saunders K;Castells-Graells R;Ferriol I;Santoni M;Steele JFC;Ranson NA;Avesani L;Lopez-Moya JJ;Lomonossoff GP
通过基于植物的表达产生植物螺旋病毒样颗粒（VLP）一直存在问题，先前的研究表明RNA支架可能是其有效产生所必需的。为了检验这一点，我们比较了当外壳蛋白从基于复制型马铃薯病毒X的载体（pEff）和非复制型载体（pEAQ-HT）表达时，来自两种马铃薯X病毒（番木瓜花叶病毒和互花米草花叶病毒（AltMV））的VLP的积累。当使用pEff时，可以纯化显著更大量的VLP。pEff系统在产生来自不同病毒家族的螺旋病毒的VLP方面也非常有效。从pEff产生的AltMV和烟草花叶病毒VLP的RNA含量的检查揭示了载体衍生的RNA序列的存在，表明复制的RNA充当VLP组装的支架。AltMV VLP的Cryo-EM分析显示它们具有与真实马铃薯X病毒颗粒非常相似的结构。因此，我们得出结论，载体产生复制形式的RNA，如pEff，是非常有效的生产螺旋VLP。
The production of plant helical virus-like particles (VLPs) via plant-based expression has been problematic with previous studies suggesting that an RNA scaffold may be necessary for their efficient production. To examine this, we compared the accumulation of VLPs from two potexviruses, papaya mosaic virus and alternanthera mosaic virus (AltMV), when the coat proteins were expressed from a replicating potato virus X- based vector (pEff) and a non-replicating vector (pEAQ-HT). Significantly greater quantities of VLPs could be purified when pEff was used. The pEff system was also very efficient at producing VLPs of helical viruses from different virus families. Examination of the RNA content of AltMV and tobacco mosaic virus VLPs produced from pEff revealed the presence of vector-derived RNA sequences, suggesting that the replicating RNA acts as a scaffold for VLP assembly. Cryo-EM analysis of the AltMV VLPs showed they had a structure very similar to that of authentic potexvirus particles. Thus, we conclude that vectors generating replicating forms of RNA, such as pEff, are very efficient for producing helical VLPs.
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影响因子：
3
作者：
Fromm, Simon A.;Bharat, Tanmay A. M.;Jakobi, Arjen J.;Hagen, Wim J. H.;Sachse, Carsten
通讯作者：
Sachse, Carsten
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13.8
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影响因子：
3.7
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通讯作者：
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