Cell behaviors within a confined adhesive area fabricated using novel micropatterning methods.
Cell behaviors within a confined adhesive area fabricated using novel micropatterning methods.
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DOI：
10.1371/journal.pone.0262632
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Yumura S
中科院分区：
文献类型：
--
作者：
Nakatoh T;Osaki T;Tanimoto S;Jahan MGS;Kawakami T;Chihara K;Sakai N;Yumura S
在细胞和组织工程领域，对空间控制细胞粘附到所需尺寸和形状的基底的技术的需求日益增加。在这里，我们描述了两种制造用于将细胞粘附到指定区域的基底的新方法。在第一种方法中，盖玻片或塑料皿的表面涂覆有Lipidure（一种非粘性涂层材料），并且通过具有孔的掩模施加空气等离子体，以赋予表面附着力。在第二种方法中，在盖玻片的表面通过溅射涂覆有金之后，然后涂覆有Lipidure;使用新型扫描激光烧蚀方法局部去除Lipidure涂层。这些方法有效地将细胞限制在粘附区域内，并且与目前可用的商业基底相比，使我们能够更长时间地跟踪单个细胞。通过在受限区域内跟踪单个细胞，我们能够观察到细胞分裂、细胞-细胞碰撞以及细胞与粘附和非粘附区域之间边界的碰撞等细胞行为的几个新方面。
In the field of cell and tissue engineering, there is an increasing demand for techniques to spatially control the adhesion of cells to substrates of desired sizes and shapes. Here, we describe two novel methods for fabricating a substrate for adhesion of cells to a defined area. In the first method, the surface of the coverslip or plastic dish was coated with Lipidure, a non-adhesive coating material, and air plasma was applied through a mask with holes, to confer adhesiveness to the surface. In the second method, after the surface of the coverslip was coated with gold by sputtering and then with Lipidure; the Lipidure coat was locally removed using a novel scanning laser ablation method. These methods efficiently confined cells within the adhesive area and enabled us to follow individual cells for a longer duration, compared to the currently available commercial substrates. By following single cells within the confined area, we were able to observe several new aspects of cell behavior in terms of cell division, cell–cell collisions, and cell collision with the boundary between adhesive and non-adhesive areas.
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DOI：
10.1073/pnas.2016602118
发表时间：
2021-02-16
影响因子：
11.1
作者：
Brückner DB;Arlt N;Fink A;Ronceray P;Rädler JO;Broedersz CP
通讯作者：
Broedersz CP
影响因子：
56.9
作者：
Piner, RD;Zhu, J;Mirkin, CA
通讯作者：
Mirkin, CA
影响因子：
9.3
作者：
Allen GM;Lee KC;Barnhart EL;Tsuchida MA;Wilson CA;Gutierrez E;Groisman A;Theriot JA;Mogilner A
通讯作者：
Mogilner A
影响因子：
4.6
作者：
Pervin MS;Itoh G;Talukder MSU;Fujimoto K;Morimoto YV;Tanaka M;Ueda M;Yumura S
通讯作者：
Yumura S
影响因子：
3.7
作者：
ABERCROMBIE, M;HEAYSMAN, JEM
通讯作者：
HEAYSMAN, JEM