Bovine gallbladder mucin promotes cholesterol crystal nucleation from cholesterol-transporting vesicles in supersaturated model bile.
Bovine gallbladder mucin promotes cholesterol crystal nucleation from cholesterol-transporting vesicles in supersaturated model bile.
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牛胆囊粘蛋白促进过饱和模型胆汁中胆固醇运输囊泡的胆固醇晶体成核。
作者：
T. Lee;Bernard F. Smith;B. F. Smith;Bovine
这项研究检查了纯化的胆囊粘蛋白加速过饱和模型胆汁中胆固醇运输颗粒中一水胆固醇晶体成核的能力。通过Sephadex G-200柱层析分离过饱和模型胆汁中的混合脂质胶束和胆固醇-磷脂酰胆碱囊泡。通过柱色谱法制备的混合脂质胶束具有较低的胆固醇-磷脂酰胆碱比(0.30)并且不会自发地使胆固醇一水合物晶体成核。相比之下，通过柱层析制备的囊泡的胆固醇-磷脂酰胆碱比为1.00，且胆固醇晶体迅速成核(P小于0.001)。纯化的牛胆囊粘蛋白在含胆固醇的囊泡中以浓度和时间依赖性方式显着加速胆固醇晶体的成核，但在混合脂质胶束中则不然（P 小于 0.001）。快速过滤结合测定表明，囊泡中的胆固醇和磷脂酰胆碱与胆囊粘蛋白显着结合，但混合胶束中的胆固醇和磷脂酰胆碱仅具有最小的结合。这些数据表明，胆囊粘蛋白与囊泡中的胆固醇和磷脂酰胆碱结合，并加速过饱和模型胆汁中这些胆固醇运输颗粒中一水胆固醇晶体的成核。
This study examined the ability of purified gallbladder mucin to accelerate the nucleation of cholesterol monohydrate crystals from the cholesterol-transporting particles in supersaturated model bile. Mixed lipid micelles and cholesterol-phosphatidylcholine vesicles in supersaturated model bile were separated by Sephadex G-200 column chromatography. Mixed lipid micelles prepared by column chromatography had a low cholesterol-phosphatidylcholine ratio (0.30) and did not spontaneously nucleate cholesterol monohydrate crystals. In contrast, vesicles prepared by column chromatography had a cholesterol-phosphatidylcholine ratio of 1.00 and nucleated cholesterol crystals rapidly (P less than 0.001). Nucleation of cholesterol crystals was significantly accelerated in a concentration- and time-dependent manner by purified bovine gallbladder mucin in cholesterol containing vesicles, but not in mixed lipid micelles (P less than 0.001). A rapid filtration binding assay demonstrated significant binding of cholesterol and phosphatidylcholine in vesicles to gallbladder mucin but only minimal binding of cholesterol and phosphatidylcholine in mixed micelles. These data indicate that gallbladder mucin binds cholesterol and phosphatidylcholine in vesicles and accelerates the nucleation of cholesterol monohydrate crystals from these cholesterol-transporting particles in supersaturated model bile.
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牛胆囊粘蛋白的疏水结合特性。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Smith,BF;LaMont,JT
通讯作者：
LaMont,JT
牛磺胆酸盐对混合脂质胶束大小的影响及其与大鼠胆汁中色素和蛋白质的关系。
影响因子：
6.5
作者：
Reuben,A;Howell,KE;Boyer,JL
通讯作者：
Boyer,JL
狗天然肝胆汁的准弹性光散射研究：与模型胆汁脂质系统的聚合行为进行比较。
影响因子：
2.9
作者：
Mazer,NA;Schurtenberg,P;Carey,MC;Preisig,R;Weigand,K;Känzig,W
通讯作者：
Känzig,W
影响因子：
2.9
作者：
MAZER, NA;CAREY, MC
通讯作者：
CAREY, MC
影响因子：
15.9
作者：
LEE, SP;LAMONT, JT;CAREY, MC
通讯作者：
CAREY, MC