Involvement of MEM1 in DNA demethylation in Arabidopsis
Involvement of MEM1 in DNA demethylation in Arabidopsis
复制标题
MEM1 参与拟南芥 DNA 去甲基化
DOI：
10.1007/s11103-019-00949-0
复制
发表时间：
2020-01
影响因子：
5.1
通讯作者：
La Honggui
中科院分区：
文献类型：
--
作者：
Lu Yanke;Dai Jie;Yang Liu;La Yumei;Zhou Shaoxia;Qiang Sheng;Wang Qianqian;Tan Feng;Wu Yufeng;Kong Weiwen;La Honggui
MEM 1参与ROS 1介导的DNA去甲基化途径，并与ROS 3一样抵消RdDM途径的影响，MEM 1突变导致拟南芥基因组中出现大量的高DMRs。在高等植物中，DNA甲基化在沉默转录基因和转座因子（TEs）中起重要作用。沉默恢复因子1（ROS 1）介导的主动DNA去甲基化能够拮抗RNA介导的DNA甲基化（RdDM）途径引起的DNA甲基化作用，对维持DNA甲基化水平具有重要作用。本研究利用Chop-PCR方法从T-DNA插入突变体群体中筛选出一个甲基化水平升高的突变体，命名为mem 1（methylation elevated mutant 1）。MEM 1具有一个Zf-C3 HC结构域，定位于细胞核中，并且在子叶中高度表达。全基因组亚硫酸氢盐测序数据显示，MEM 1的敲除突变导致4519个CG、1793个CHG和12739个CHH超DMR（对于差异甲基化区域）。进一步分析表明，mem 1 - 1与3个突变体（ros 1 -4、rdd和ros 3 -2）之间分别存在2751、2216和2042个重叠的CG hyper-DMR，与3个突变体（ros 1 -4、rdd和ros 3 -2）之间分别存在797、2514和6766个重叠的CHH hyper-DMR; mem 1 nrpd 1 -3和mem 1 rdm 1双突变体在4个测试位点上显示几乎完全或部分的超甲基化缺失，这表明MEM 1在抵消过度的DNA甲基化方面具有与DNA糖基化酶/裂解酶类似的功能，MEM 1作为沉默抑制因子3（ROS 3）在消除RdDM途径引起的CHH甲基化中起重要作用。总之，这些数据表明MEM 1参与ROS 1介导的DNA去甲基化途径以及MEM 1和ROS 3之间的功能连接。
MEM1 participates in ROS1-mediated DNA demethylation pathway, and acts functionally as ROS3 to counteract the effects of RdDM pathway.mem1mutation leads to large numbers of hyper-DMRs inArabidopsisgenome. In higher plants, DNA methylation performs important functions in silencing transcribed genes and transposable elements (TEs). Active DNA demethylation mediated by REPRESSOR OF SILENCING 1 (ROS1) is able to antagonize the action of DNA methylation caused by RNA-directed DNA methylation (RdDM) pathway, which plays critical roles in keeping DNA methylation at a proper level. In this study, a new mutant named mem1 (for methylation elevated mutant 1) was isolated from a genetic screen of T-DNA insertional mutant population for lines with elevated DNA methylation at a particular locus through Chop-PCR method. MEM1 possesses a Zf-C3HC domain, and is localized in nucleus as well as highly expressed in cotyledons. Whole-genome bisulfite sequencing data showed that knockout mutation of MEM1 leads to 4519 CG, 1793 CHG and 12739 CHH hyper-DMRs (for differentially methylated regions). Further analysis indicated that there are 2751, 2216 and 2042 overlapped CG hyper-DMRs between mem1-1and three mutants, i.e. ros1-4, rdd and ros3-2, respectively; 797, 2514, and 6766 overlapped CHH hyper-DMRs were observed between mem1-1 and three such mutants, respectively; mem1 nrpd1-3 and mem1 rdm1 double mutants showed nearly complete or partial loss of hypermethylation at 4 tested loci, suggesting that MEM1 performs similar functions as DNA glycosylase/lyases in counteracting excessive DNA methylation, and MEM1 plays important roles as REPRESSOR OF SILENCING 3 (ROS3) in erasing CHH methylation caused by the RdDM pathway. Together, these data demonstrate the involvement of MEM1 in ROS1-mediated DNA demethylation pathway and functional connections between MEM1 and ROS3.
登录
查看更多内容
影响因子：
4.5
作者：
Li Y;Córdoba-Cañero D;Qian W;Zhu X;Tang K;Zhang H;Ariza RR;Roldán-Arjona T;Zhu JK
通讯作者：
Zhu JK
影响因子：
6.9
作者：
Qianqian Wang;Q. Guo;Yuanyuan Guo;Jieshu Yang;Min Wang;Xiaoke Duan;Jiayu Niu;Shuai Liu;Jianzhen Zhang;Yanke Lu;Zhi-hui Hou;W. Miao;Xiangyu Wang;Weiwen Kong;Xiaoming Xu;Yufeng Wu;Q. Rui;Honggui La
通讯作者：
Qianqian Wang;Q. Guo;Yuanyuan Guo;Jieshu Yang;Min Wang;Xiaoke Duan;Jiayu Niu;Shuai Liu;Jianzhen Zhang;Yanke Lu;Zhi-hui Hou;W. Miao;Xiangyu Wang;Weiwen Kong;Xiaoming Xu;Yufeng Wu;Q. Rui;Honggui La
影响因子：
64.8
作者：
Zheng, Xianwu;Pontes, Olga;Zhu, Jianhua;Miki, Daisuke;Zhang, Fei;Li, Wen-Xue;Iida, Kei;Kapoor, Avnish;Pikaard, Craig S.;Zhu, Jian-Kang
通讯作者：
Zhu, Jian-Kang
影响因子：
16
作者：
Martínez-Macías MI;Qian W;Miki D;Pontes O;Liu Y;Tang K;Liu R;Morales-Ruiz T;Ariza RR;Roldán-Arjona T;Zhu JK
通讯作者：
Zhu JK
影响因子：
18
作者：
通讯作者：
--