Structure and flexibility of the complete periplasmic domain of BamA: the protein insertion machine of the outer membrane.
Structure and flexibility of the complete periplasmic domain of BamA: the protein insertion machine of the outer membrane.
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DOI：
10.1016/j.str.2010.08.012
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发表时间：
2010-11-10
期刊：
影响因子：
--
通讯作者：
Sousa MC
中科院分区：
文献类型：
--
作者：
Gatzeva-Topalova PZ;Warner LR;Pardi A;Sousa MC
β-桶状外膜蛋白（OMPs）的折叠和插入对革兰氏阴性菌至关重要。这一过程由多蛋白复合物BAM介导，BAM由必需的β-桶状外膜蛋白BamA和四种脂蛋白（BamBCDE）组成。BamA的周质结构域对其功能至关重要，包含五个“多肽转运相关”（POTRA）重复序列。在此我们报道了POTRA4 - 5串联结构的晶体结构，其中包含对BAM复合物形成和细胞活力必需的POTRA5。晶体中观察到的结构域取向通过溶液核磁共振（NMR）和小角X射线散射（SAXS）得到验证。利用先前确定的BamA POTRA1 - 4结构，我们提出了一个经小角X射线散射验证的整个BamA周质结构域的拼接模型。溶液散射表明，POTRA2和3之间的构象灵活性产生了紧凑和伸展的构象。BamA在其伸展构象下的长度表明，该蛋白可能横跨周质空间连接内膜和外膜。
Folding and insertion of β-barrel outer membrane proteins (OMPs) is essential for Gram-negative bacteria. This process is mediated by the multiprotein complex BAM, composed of the essential β-barrel OMP BamA and four lipoproteins (BamBCDE). The periplasmic domain of BamA is key for its function and contains five “polypeptide transport-associated” (POTRA) repeats. Here we report the crystal structure of the POTRA4-5 tandem, containing the essential for BAM complex formation and cell viability POTRA5. The domain orientation observed in the crystal is validated by solution NMR and SAXS. Using previously determined structures of BamA POTRA1-4 we present a spliced model of the entire BamA periplasmic domain validated by SAXS. Solution scattering shows that conformational flexibility between POTRA2 and 3 gives rise to compact and extended conformations. The length of BamA in its extended conformation suggests that the protein may bridge the inner and outer membranes across the periplasmic space.
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影响因子：
6.1
作者：
Konarev, PV;Volkov, VV;Svergun, DI
通讯作者：
Svergun, DI
影响因子：
56.9
作者：
Kim, Seokhee;Malinverni, Juliana C.;Kahne, Daniel
通讯作者：
Kahne, Daniel
影响因子：
6.1
作者：
Kozin, MB;Svergun, DI
通讯作者：
Svergun, DI
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
2.7
作者：
DELAGLIO, F;GRZESIEK, S;BAX, A
通讯作者：
BAX, A