Comparative Analysis of Gene Expression Profiles of Human Dental Fluorosis and Kashin-Beck Disease.
Comparative Analysis of Gene Expression Profiles of Human Dental Fluorosis and Kashin-Beck Disease.
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DOI：
10.1038/s41598-017-18519-z
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发表时间：
2018-01-09
影响因子：
4.6
通讯作者：
Bai S
中科院分区：
文献类型：
--
作者：
Zhang Q;Ma J;Liu H;He D;Chen L;Wu H;Jiang H;Lu Q;Bai S
为探讨大骨节病（KBD）和大骨节病合并氟斑牙（DF）的病理机制，我们对基因表达谱进行了比较分析。入选受试者12例，其中大骨节病患者4例，大骨节病合并DF患者4例，健康对照4例。通过定制的寡核苷酸芯片评估其外周血单个核细胞的全基因组表达谱。使用R编程软件进行微阵列数据分析，然后通过KOBAS进行功能富集分析。鉴定了几种潜在的生物标志物，并使用定量实时逆转录-聚合酶链反应（qRT-PCR）对其进行验证。本研究发现，与健康人相比，大骨节病患者中有28个基因表达上调，8个基因表达下调。在大骨节病和DF患者中，与健康对照相比，我们获得了10个上调和3个下调的基因。值得注意的是，在两组患者之间没有发现差异表达基因（DEG）。在大骨节病患者和大骨节病合并DF患者的DEG之间共获得10个重叠（DUSP 2、KLRF 1、SRP 19、KLRC 3、CD 69、SIK 1、ITGA 4、ID 3、HSPA 1A、GPR 18）。它们在代谢、分化、凋亡和骨发育中起重要作用。通过qRT-PCR分析进一步证实了8个DEG的相对丰度，即FCRL 6、KLRC 3、CXCR 4、CD 93、CLK 1、GPR 18、SRP 19和KLRF 1。
To explore the pathologies of Kashin-Beck disease (KBD) and KBD accompanied with dental fluorosis (DF), we conducted a comparative analysis of gene expression profiles. 12 subjects were recruited, including 4 KBD patients, 4 patients with KBD and DF and 4 healthy subjects. Genome-wide expression profiles from their peripheral blood mononuclear cells were evaluated by customized oligonucleotide microarray. R programming software was used for the microarray data analysis followed by functional enrichment analysis through KOBAS. Several potential biomarkers were identified, and quantitative real-time reverse transcription–polymerase chain reaction (qRT-PCR) was used for their validation. In this study, 28 genes and 8 genes were found to be up- and down-regulated respectively in KBD patients compared with health subjects. In patients with KBD and DF, we obtained 10 up-regulated and 3 down-regulated genes compared with health controls. Strikingly, no differential expression gene (DEG) was identified between the two groups of patients. A total of 10 overlaps (DUSP2, KLRF1, SRP19, KLRC3, CD69, SIK1, ITGA4, ID3, HSPA1A, GPR18) were obtained between DEGs of patients with KBD and patients with KBD and DF. They play important roles in metabolism, differentiation, apoptosis and bone-development. The relative abundance of 8 DEGs, i.e. FCRL6, KLRC3, CXCR4, CD93, CLK1, GPR18, SRP19 and KLRF1, were further confirmed by qRT-PCR analysis.
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影响因子：
4.8
作者：
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通讯作者：
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通讯作者：
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蛋白多糖代谢、细胞死亡和大骨节病
影响因子：
3
作者：
Li, Siyuan;Cao, Junling;Caterson, Bruce;Hughes, Clare E.
通讯作者：
Hughes, Clare E.