Tuning the Mechanical and Geometric Properties of Electrochemically Aligned Collagen Threads Towards Applications in Biohybrid Robotics.
Tuning the Mechanical and Geometric Properties of Electrochemically Aligned Collagen Threads Towards Applications in Biohybrid Robotics.
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调整电化学排列的胶原线的机械和几何特性以适应生物混合机器人技术的应用。
DOI：
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Victoria A. Webster
中科院分区：
文献类型：
--
作者：
Wenhuan Sun;Jason M. Paulovich;Victoria A. Webster
通过胶原蛋白等电聚焦（IF）制造的电化学排列胶原蛋白（ELAC）线先前已在组织工程以及最近在生物混合机器人结构的制造中显示出潜力。对于生物混合机器人的应用，需要足够坚固且柔顺的 ELAC 结构，以促进肌肉驱动。然而，之前的文献中尚未发现有关 IF 参数的影响以及此类制造参数的相互作用对所得 ELAC 螺纹的机械和几何性能的研究。了解这些制造参数对材料特性的影响对于促进生物混合机器人设计至关重要。在这项研究中，研究了 IF 持续时间、IF 电压和胶原蛋白溶液浓度的影响，并通过单因素实验显示出对调整 ELAC 特性具有统计显着性的影响。通过双因素实验，参数之间的相互作用对 ELAC 性能调整表现出显着的联合效应。扫描电子显微镜和 2,4,6-三硝基苯磺酸测定揭示了高机械性能与低孔隙率和高交联度的组合之间的相关性。通过简单地调整 IF 参数而不改变其他制造步骤（例如交联剂浓度），就可以制造出具有广泛机械和几何特性的 ELAC 线。所得 ELAC 线的平均拉伸模量范围为 198±90 至 758±138 MPa。平均横截面积范围为 7756±1000 至 1775±457 µm2。 IF 参数和 ELAC 螺纹属性之间的最终映射使得能够生产具有可定制属性的强大而灵活的螺纹。
Electrochemically Aligned Collagen (ELAC) threads fabricated by the isoelectric focusing (IF) of collagen have previously shown potential in tissue engineering and more recently in the fabrication of biohybrid robot structures. For applications in biohybrid robotics, ELAC structures are needed that are both robust and compliant enough to facilitate muscle actuation. However, studies on the effects of IF parameters, and the interactions of such fabrication parameters, on the mechanical and geometric properties of resulting ELAC threads have not been previously found in literature. Understanding the impact of these manufacturing parameters on the material properties is critical to facilitate biohybrid robot design. In this study, the effects of IF duration, IF voltage, and collagen solution concentration were investigated and showed statistically significant effects on adjusting ELAC properties via single-factor experiments. The interactions between parameters exhibited significant joint effects on ELAC property tuning through two-factor experiments. Scanning electron microscopy and 2,4,6-trinitrobenzenesulfonic assays revealed the correlation between high mechanical properties and a combination of low porosity and high degree of crosslinking. By simply tuning IF parameters without changing other fabrication steps, such as crosslinker concentration, ELAC threads with a wide range of mechanical and geometric properties were fabricated. The average tensile modulus of the resulting ELAC threads ranged from 198±90 to 758±138 MPa. The average cross-sectional area ranged from 7756±1000 to 1775±457 µm2. The resultant mapping between IF parameters and ELAC thread properties enabled the production of strong and flexible threads with customizable properties.
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影响因子：
--
作者：
Barnes, Catherine P.;Pemble, Charles W.;Bowlin, Gary L.
通讯作者：
Bowlin, Gary L.
DOI：
10.1002/jbm.b.31962
发表时间：
2012-02-01
影响因子：
3.4
作者：
Kishore, Vipuil;Uquillas, Jorge Alfredo;Akkus, Ozan
通讯作者：
Akkus, Ozan
DOI：
10.1002/jbm.b.34279
发表时间：
2019-08-01
影响因子：
3.4
作者：
Learn, Greg D.;McClellan, Phillip E.;Akkus, Ozan
通讯作者：
Akkus, Ozan
影响因子：
4.9
作者：
Gurkan, Umut Atakan;Cheng, Xingguo;Kishore, Vipuil;Uquillas, Jorge Alfredo;Akkus, Ozan
通讯作者：
Akkus, Ozan
影响因子：
9.7
作者：
Kishore, Vipuil;Paderi, John E.;Akkus, Anna;Smith, Katie M.;Balachandran, Dave;Beaudoin, Stephen;Panitch, Alyssa;Akkus, Ozan
通讯作者：
Akkus, Ozan