Impact of maternal diabetes exposure on soluble adhesion molecules in the offspring.
Impact of maternal diabetes exposure on soluble adhesion molecules in the offspring.
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DOI：
10.1016/j.numecd.2022.01.034
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发表时间：
2022-05
期刊：
影响因子：
--
通讯作者：
Tryggestad JB
中科院分区：
文献类型：
--
作者：
Landreth S;Teague AM;Jensen ME;Gulati S;Tryggestad JB
可溶性粘附分子与心血管疾病有关，在糖尿病患者中增加。本研究评估了子宫内糖尿病暴露对脐带血清中循环黏附分子丰度的影响，以及暴露于高葡萄糖浓度的人脐静脉内皮细胞（HUVEC）释放的可溶性黏附分子。研究人员招募了患有和不患有糖尿病的女性。糖尿病的诊断依据是美国糖尿病协会的标准。HUVEC原代培养分别在5mM和25mM葡萄糖中培养，并采用25mM甘露醇渗透控制。采用ELISA法测定脐带血血清和条件HUVEC培养基中可溶性粘附分子、细胞内粘附分子（ICAM）、血管细胞粘附分子（VCAM）和e -选择素的含量。DM母亲年龄较大，BMI较高（p=0.027、0.008）。在完全调整模型中，糖尿病母亲所生婴儿脐带血清中VCAM显著升高（p=0.046），但ICAM和E-selectin无显著差异。ICAM与母亲HbA1c （r2=0.16, p=0.004）和脐带血清非酯化脂肪酸（r2=0.08, p=0.013）也显著相关。从HUVEC培养基中，黏附分子的丰度没有因DM或高糖暴露而不同；而HUVEC上清中VCAM丰度与ICAM （r2=0.27, p=0.010）和脐带血清c肽（r2= 0.19, p=0.043）显著相关。妊娠期暴露于糖尿病环境的婴儿可溶黏附分子丰度的改变可能反映了早期血管功能的改变，可预测未来的心血管疾病。
Soluble adhesion molecules are associated with cardiovascular disease and increased in individuals with diabetes. This study assesses the impact of diabetes exposure in utero on the abundance of circulating adhesion molecules in cord serum and soluble adhesion molecules released from human umbilical vein endothelial cells (HUVEC) exposed to high glucose concentrations. Women with and without diabetes were recruited. DM was diagnosed based on the American Diabetes Association criteria. Primary cultures of HUVEC were cultured in 5mM and 25mM glucose with 25mM mannitol osmotic control. The soluble adhesion molecules, intracellular adhesion molecule (ICAM), vascular cell adhesion molecule (VCAM) and E-selectin were measured by ELISA in the cord blood serum and conditioned HUVEC media. The mothers with DM were older with higher BMI (p=0.027 and 0.008, respectively). In a fully adjusted model, VCAM was significantly increased in the cord serum of infants born to mothers with diabetes (p=0.046), but ICAM and E-selectin were not different. ICAM was also significantly correlated with maternal HbA1c (r2=0.16, p=0.004) and cord serum non-esterified fatty acids (r2=0.08, p=0.013). From the HUVEC media, the abundance of adhesion molecules was not different based on DM or high glucose exposure; however, VCAM abundance in the HUVEC supernatant was significantly correlated with ICAM (r2=0.27, p=0.010) and cord serum c-peptide (R2=0.19, p=0.043). Alterations in soluble adhesion molecule abundance in infants exposed to the diabetic milieu of pregnancy may reflect early alterations in vascular function predicting future cardiovascular disease.
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影响因子：
3.1
作者：
Fukami, Kei;Yamagishi, Sho-ichi;Okuda, Seiya
通讯作者：
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影响因子：
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作者：
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通讯作者：
Humes, HD
影响因子：
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作者：
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