Characterization of murine thymocytes with CDS-associated T-cell receptor structures
Characterization of murine thymocytes with CDS-associated T-cell receptor structures
复制标题
小鼠胸腺细胞与 CDS 相关 T 细胞受体结构的表征
作者：
J. Bluestone;D. Pardoll;S. Sharrow;B. Fowlkes
胸腺是T细胞受体（TCR）基因重排和T细胞成熟的主要场所1，2。鼠T细胞上的特异性抗原识别结构（TCR）已被证明依赖于一组多态性的二硫键连接异二聚体，包含两个完整的膜糖蛋白链TCRα和TCRβ，与不变的蛋白质复合物CD 3（T3）3-6以非共价结合的方式表达。最近，一种新的TCR/CD 3复合物，包括TCRγ基因的产物，已经在外周细胞和胸腺细胞的亚群上被鉴定7 -9。在这里，我们研究的表达TCR/CD 3复合物在胎儿个体发育和成人胸腺。结果表明，CD 3 +4−8−（T3+，L3 T4 −，Lyt 2 −）细胞在第15天的胎儿胸腺，整个胎儿发育和成人胸腺中检测到。原位杂交研究表明，这些早期CD 3+细胞表达高水平的TCRγ特异性RNA，低水平的TCRβ特异性RNA，并且没有可检测到的TCRα特异性RNA。在存在抗CD 3单克隆抗体和白细胞介素-2（IL-2）的情况下培养时，第16天CD 3+、4−、8−胎胸腺细胞可被激活增殖并显示出溶细胞活性。这些结果表明，CD 3-轴承细胞，目前在胸腺个体发育早期，表达功能TCR，因此，可能是重要的剧目发展。
The thymus is the major site for T-cell receptor (TCR) gene rearrangement and T-cell maturation1,2. The specific antigen recognition structure (TCR) on murine T cells has been shown to be dependent on a polymorphic set of disulphide-linked heterodimers, containing two integral membrane glycoprotein chains, TCRα and TCRβ, expressed in non-covalent association with an invariant complex of proteins, CD3 (T3)3–6. Recently, a novel TCR/CD3 complex, that includes the product of the TCRγ gene, has been identified on a subset of both peripheral cells and thymocytes7–9. Here we examine the expression of TCR/CD3 complexes in fetal ontogeny and in the adult thymus. The results demonstrate that CD3+4−8−(T3+, L3T4−, Lyt2−) cells are detected in day-15 fetal thymi, throughout fetal development and in adult thymus. In situ hybridization studies indicate that these early CD3+ cells express high levels of TCRγ-specific RNA, low levels of TCRβ-specific RNA and no detectable TCRα-specific RNA. Day-16 CD3+, 4−, 8− fetal thymocytes can be activated to proliferate and demonstrate cytolytic activity when cultured in the presence of anti-CD3 monoclonal antibodies and interleukin-2 (IL-2). These results suggest that CD3-bearing cells, present early in thymic ontogeny, express a functional TCR and may, therefore, be important in repertoire development.
影响因子：
4.4
作者：
James P. Allison;Bradley W. McIntyre;D. Bloch
通讯作者：
James P. Allison;Bradley W. McIntyre;D. Bloch